Manikkam V, Mathai M L, Street W A, Donkor O N, Vasiljevic T
Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, P.O. BOX 14428, Melbourne, VIC 8001 Australia.
Center for Chronic Diseases, College of Health and Biomedicine, Victoria University, P.O. BOX 14428, Melbourne, VIC 8001 Australia.
J Food Sci Technol. 2016 Sep;53(9):3574-3582. doi: 10.1007/s13197-016-2338-1. Epub 2016 Sep 13.
Australian underutilised fish species may serve as a potential source of valuable proteins and potent bioactive peptides. This novel research is the first to investigate the effects of storage-processing conditions and an in-vitro simulated gastrointestinal digestion (pepsin-pancreatin) on bioactive peptides' release during storage of fish fillet, derived from Australian silver warehou (). In-vitro bioactivities including angiotensin-converting enzyme and trypsin inhibitory and antioxidant activities were analysed. The antioxidant power was evaluated by DPPH free radical scavenging activity, Cu chelating and Fe reducing abilities. Fillets were stored at chilled (4 and 6 °C) and freezing (-18 °C) temperatures for 7 and 28 days, respectively. Results indicated that during postmortem storage, endogenous enzymes released from fillets an array of polypeptides during storage. The demonstrated physiological activities were further increased during simulated digestion. Bioactivities were greater at 4 °C, increasing over 7 days as compared to at 6 and -18 °C. An increase by 2 °C for chilled temperature was enough to cause significant changes in activities. The crude extracts obtained by pancreatin treatment demonstrated the highest metal chelating activities at 4 °C (86.3 ± 0.1 % on day 7). Physiological potency, especially metal chelating activity, of fillets obtained from silver warehou may be manipulated by storage conditions that would consequently be further enhanced during simulated digestion.
澳大利亚未被充分利用的鱼类品种可能是有价值蛋白质和强效生物活性肽的潜在来源。这项新研究首次调查了储存加工条件以及体外模拟胃肠消化（胃蛋白酶 - 胰蛋白酶）对源自澳大利亚银纹笛鲷（）鱼片储存期间生物活性肽释放的影响。分析了包括血管紧张素转换酶、胰蛋白酶抑制和抗氧化活性在内的体外生物活性。通过DPPH自由基清除活性、铜螯合和铁还原能力评估抗氧化能力。鱼片分别在冷藏（4和6°C）和冷冻（-18°C）温度下储存7天和28天。结果表明，在死后储存期间，鱼片释放的内源性酶在储存过程中产生了一系列多肽。在模拟消化过程中，所展示的生理活性进一步增强。4°C时生物活性更高，与6°C和-18°C相比，在7天内有所增加。冷藏温度升高2°C就足以导致活性发生显著变化。胰蛋白酶处理获得的粗提物在4°C时表现出最高的金属螯合活性（第7天为86.3±0.1%）。银纹笛鲷鱼片的生理效力，尤其是金属螯合活性，可能会受到储存条件的影响，而在模拟消化过程中会进一步增强。
