Shao Cuijie, Duan Changsheng, Wang Jiani, Luan Shunlian, Gao Yong, Jin Dan, Wang Deqiang, Li Yuming, Xu Lihua
Department of Pain, Affiliated Hospital of Binzhou Medical College, Binzhou, 256600 China.
Department of Gastrointestinal Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou, 256600 China.
Cancer Cell Int. 2016 Oct 10;16:79. doi: 10.1186/s12935-016-0357-7. eCollection 2016.
The molecular mechanisms underlying the development and progression of gastric carcinoma remain poorly understood. The main objective of this study was to investigate the expression level of targeting protein for Xenopus kinesin-like protein 2 (TPX2) and its clinical significance in human gastric carcinoma.
Real-time quantitative polymerase chain reaction (RT-PCR) and western blotting were used to determine the mRNA and protein levels of TPX2 in 20 paired gastric carcinoma tissues and the adjacent normal tissues, and the expression of TPX2 protein in 106 specimens of a gastric carcinoma tissue microarray was determined by immunohistochemistry. The associations of TPX2 expression with the clinicopathological features were analyzed, and the prognosis of gastric carcinoma patients was evaluated.
The results showed that the expression of TPX2 mRNA was significantly higher in gastric carcinoma than in the adjacent normal tissues in 20 paired samples. Western blotting analysis revealed that TPX2 protein was differentially increased in 17 of 20 specimens from primary human gastric carcinoma tissues compared with those from adjacent non-tumor tissues. Immunohistochemical staining showed that TPX2 over-expression was significantly associated with advanced age (P = 0.001) and tumor T stage (P = 0.003). In addition, TPX2 was an independent prognostic factor for overall survival (OS) in the multivariate analysis [hazard ratio (HR) 0.001; 95 % confidence interval (CI) 2.626-7.198; P = 0.001].
TPX2 is up-regulated in gastric carcinoma and is associated with old age and tumor T stage. TPX2 may serve as a good prognostic indicator in patients with gastric carcinoma.
胃癌发生和发展的分子机制仍未完全明确。本研究的主要目的是探讨非洲爪蟾驱动蛋白样蛋白2靶向蛋白（TPX2）在人胃癌中的表达水平及其临床意义。
采用实时定量聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测20对胃癌组织及其癌旁正常组织中TPX2的mRNA和蛋白水平，并用免疫组织化学法检测106例胃癌组织芯片标本中TPX2蛋白的表达。分析TPX2表达与临床病理特征的相关性，并评估胃癌患者的预后。
结果显示，在20对样本中，胃癌组织中TPX2 mRNA的表达明显高于癌旁正常组织。蛋白质免疫印迹分析显示，与癌旁非肿瘤组织相比，20例原发性人胃癌组织标本中有17例TPX2蛋白表达差异增加。免疫组织化学染色显示，TPX2过表达与高龄（P = 0.001）和肿瘤T分期（P = 0.003）显著相关。此外，在多因素分析中，TPX2是总生存期（OS）的独立预后因素[风险比（HR）0.001；95%置信区间（CI）2.626 - 7.198；P = 0.001]。
TPX2在胃癌中上调，与高龄和肿瘤T分期相关。TPX2可能是胃癌患者的一个良好预后指标。
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