TPX2的表达与胃癌患者的不良预后相关。
TPX2 expression is associated with poor survival in gastric cancer.
作者信息
Tomii Chiharu, Inokuchi Mikito, Takagi Yoko, Ishikawa Toshiaki, Otsuki Sho, Uetake Hiroyuki, Kojima Kazuyuki, Kawano Tatsuyuki
机构信息
Department of Gastrointestinal Surgery, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan.
Department of Surgical Specialties, Tokyo Medical and Dental University, Tokyo, Japan.
出版信息
World J Surg Oncol. 2017 Jan 9;15(1):14. doi: 10.1186/s12957-016-1095-y.
BACKGROUND
Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells. Several studies have demonstrated that TPX2 is overexpressed in multiple tumor types and promotes tumor growth and metastasis. However, there have been few reports regarding its role in gastric cancer. In this study, we evaluated TPX2 expression and investigated its correlations with gastric cancer clinicopathological features and prognosis.
METHODS
Tumor samples were obtained from 290 patients with gastric adenocarcinoma who had undergone gastrectomy. The expression of TPX2 protein was examined using immunohistochemical staining. TPX2 messenger RNA (mRNA) levels were evaluated using real-time quantitative reverse transcription PCR in 19 of the gastric cancer tumors and adjacent normal tissues.
RESULTS
The mRNA levels of TPX2 were significantly higher in gastric cancer tissues than in matched adjacent normal tissues (p = 0.004). In the immunohistochemical analysis, TPX2 overexpression was found in 123 (42.4%) of 290 patients. High TPX2 expression was positively associated with age, type of histology, depth of tumor, lymph node metastasis, stage, and remote metastasis or recurrence. High TPX2 expression was significantly associated with poorer disease-specific survival (p = 0.004) and relapse-free interval (p = 0.013).
CONCLUSIONS
Our results indicated that high TPX2 expression was associated with tumor progression and poor survival in gastric cancer.
背景
非洲爪蟾驱动蛋白样蛋白2靶向蛋白（TPX2）是一种人类细胞中微管形成所需的微管相关蛋白。多项研究表明，TPX2在多种肿瘤类型中过表达，并促进肿瘤生长和转移。然而，关于其在胃癌中的作用报道较少。在本研究中，我们评估了TPX2的表达，并研究了其与胃癌临床病理特征及预后的相关性。
方法
从290例行胃切除术的胃腺癌患者中获取肿瘤样本。采用免疫组织化学染色检测TPX2蛋白的表达。在19例胃癌肿瘤及相邻正常组织中，运用实时定量逆转录PCR评估TPX2信使核糖核酸（mRNA）水平。
结果
胃癌组织中TPX2的mRNA水平显著高于配对的相邻正常组织（p = 0.004）。免疫组织化学分析显示，290例患者中有123例（42.4%）存在TPX2过表达。TPX2高表达与年龄、组织学类型、肿瘤深度、淋巴结转移、分期以及远处转移或复发呈正相关。TPX2高表达与较差的疾病特异性生存率（p = 0.004）和无复发生存期（p = 0.013）显著相关。
结论
我们的结果表明，TPX2高表达与胃癌的肿瘤进展及不良生存相关。
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