Burnett D, Wood S M, Bradwell A R
Biochim Biophys Acta. 1976 Mar 18;427(1):231-7. doi: 10.1016/0005-2795(76)90299-3.
The effective hydrodynamic (Stokes) radii of fifteen serum proteins were estimated by Sephadex G 200 gel filtration and immunochemical methods. The Waldmann-Meyer and Birch equation (Protides Biol. Fluids, Proc. 21st Colloq. (1974) (Peeters, H., ed.), Vol. 21, pp. 653-656, Pergamon Press, Oxford) was used for maximum accuracy. Three replicate column runs were made to assess the precision for the size determinations (coefficients of variation 0.6-2.7(). Quantive two-dimensional immunoelectrophoresis was used to measure eleven proteins in specimens of serum and amniotic fluid collected from twelve normal pregnancies. There was a close inverse linear relationship between the amniotic fluid/serum ratios of the proteins and their Stokes radii. This indicates that the movement of a protein from the blood to amniotic fluid is determined by the size of the protein. The linear correlation of protein amniotic fluid/serum ratio with Stokes radius was better than that with molecular weight. This demonstrates that, as an expression of protein size, Stokes radius should be used in preference to molecular weight when protein filtration systems are being investigated.
采用葡聚糖G 200凝胶过滤法和免疫化学方法对15种血清蛋白的有效流体动力学（斯托克斯）半径进行了估算。为获得最高精度，使用了Waldmann-Meyer和Birch方程（《生物流体中的蛋白质，第21届学术讨论会论文集》（1974年）（彼得斯，H.编），第21卷，第653 - 656页，帕格门出版社，牛津）。进行了三次重复柱实验以评估尺寸测定的精度（变异系数为0.6 - 2.7%）。采用定量二维免疫电泳法对从12例正常妊娠中采集的血清和羊水样本中的11种蛋白质进行了测定。蛋白质的羊水/血清比值与其斯托克斯半径之间存在密切的负线性关系。这表明蛋白质从血液向羊水的转运取决于蛋白质的大小。蛋白质羊水/血清比值与斯托克斯半径的线性相关性优于其与分子量的线性相关性。这表明，在研究蛋白质过滤系统时，作为蛋白质大小的一种表达方式，斯托克斯半径应优先于分子量使用。
