人源 M1 和 M2 极化巨噬细胞对纳米颗粒的摄取差异：蛋白质冠作为关键决定因素。
Differential uptake of nanoparticles by human M1 and M2 polarized macrophages: protein corona as a critical determinant.
机构信息
Department of Biomaterials Science & Technology, Targeted Therapeutics Section, MIRA Institute for Biomedical Technology & Technical Medicine, University of Twente, Enschede, The Netherlands.
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
出版信息
Nanomedicine (Lond). 2016 Nov;11(22):2889-2902. doi: 10.2217/nnm-2016-0233. Epub 2016 Oct 26.
AIM
To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum.
MATERIALS & METHODS: THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies.
RESULTS
Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses.
CONCLUSION
The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.
目的
研究有无血清存在时，不同大小的纳米颗粒与 M1 型和 M2 型巨噬细胞的相互作用行为。
材料与方法
将 THP-1 人单核细胞分化为 M1 和 M2 巨噬细胞，并用流式细胞术和不同显微镜研究了二氧化硅纳米颗粒（50-1000nm）的摄取。
结果
无血清时，M1 比 M2 摄取所有大小的纳米颗粒都多。有血清时，M2 对（200-1000nm）纳米颗粒的摄取显著增加。此外，通过 SDS-PAGE 和基因谱分析发现，纳米颗粒吸附的（冠状）血清蛋白是 M2 表达的受体的配体。
结论
M1 和 M2 巨噬细胞摄取的差异有助于理解纳米颗粒在体内的命运。
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