Department of Biomaterials Science & Technology, Targeted Therapeutics Section, MIRA Institute for Biomedical Technology & Technical Medicine, University of Twente, Enschede, The Netherlands.
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Nanomedicine (Lond). 2016 Nov;11(22):2889-2902. doi: 10.2217/nnm-2016-0233. Epub 2016 Oct 26.
To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum.
MATERIALS & METHODS: THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies.
Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses.
The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.
研究有无血清存在时，不同大小的纳米颗粒与 M1 型和 M2 型巨噬细胞的相互作用行为。
将 THP-1 人单核细胞分化为 M1 和 M2 巨噬细胞，并用流式细胞术和不同显微镜研究了二氧化硅纳米颗粒（50-1000nm）的摄取。
无血清时，M1 比 M2 摄取所有大小的纳米颗粒都多。有血清时，M2 对（200-1000nm）纳米颗粒的摄取显著增加。此外，通过 SDS-PAGE 和基因谱分析发现，纳米颗粒吸附的（冠状）血清蛋白是 M2 表达的受体的配体。
M1 和 M2 巨噬细胞摄取的差异有助于理解纳米颗粒在体内的命运。
