Han A Lum, Park Seong-Hoon, Park Mi Sung
Department of Family Medicine, Medical Hospital, Wonkwang University, Iksan 54538, Republic of Korea.
Department of Radiology Medicine, Medical Hospital, Wonkwang University, Iksan 54538, Republic of Korea.
J Microbiol Biotechnol. 2017 Feb 28;27(2):365-371. doi: 10.4014/jmb.1608.08011.
Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of β-galactosidase (an indicator of aging), and promote cell proliferation. The accumulation of β-galactosidase in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and anti-senescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.
氢作为一种抗氧化剂在预防和治疗方面具有应用潜力。然而，水中的氢微粒和大颗粒会随着时间的推移而轻易消失。为了消除与衰老过程相关的活性氧（ROS），我们使用了含有纳米颗粒氢的功能水。纳米颗粒氢不容易消失，并且在长时间处于水下时也不会分解。我们使用了从C57BL/6小鼠12.5天胚胎中分离出的小鼠胚胎成纤维细胞。我们研究了水中纳米颗粒氢抑制羟基脲诱导的ROS产生、细胞毒性以及β-半乳糖苷酶（衰老指标）积累并促进细胞增殖的能力。通过每日用氢水处理细胞，可抑制β-半乳糖苷酶在细胞质中的积累以及异常细胞核的出现。当羟基脲诱导的氧化应激加速衰老过程时，氢水的效果更加显著。因此，本研究显示了氢水的抗氧化和抗衰老作用。纳米颗粒氢水可能是一种有效的抗衰老剂。
