Wang Xiao-Ning, Yao Jian-Na, Liu Fang, Wang Xiao-Juan, Ma Juan-Juan, Guo Cai-Li, Liu Hua-Sheng, He Peng-Cheng, Zhang Mei, Sun Chun-Hong
Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Oct;24(5):1529-1532. doi: 10.7534/j.issn.1009-2137.2016.05.044.
To observe the efficacy and adverse reactions of autologous PBSC collection when the autoPBSC procedure and MNC procedure of COBE Spectra cell separator and the MNC procedure of Spectra Optia cell separator were used.
The autologous perepheral blood hematopoietic stem cells from 41 patients were collected by using autoPBSC procedure and MNC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator. The numbers of MNC and CD34 cells collected by 3 collected procedure, the difference of hemoglobin (Hb) drop and platelet decrease, and the adverse reaction of patients were observed.
When the whole blood processing and the collection time were basically same among these 3 groups, the MNC counts collected by MNC procedure of COBE Spectra and Spectra Optia were higher than that of AutoPBSC procedure of COBE Spctra, but the CD34 cell count was lower than that collected by AutoPBSC procedure (P< 0.05). The final product volume collected by MNC procedure of COBE Spectra and Spectra Optia was bigger than that collected by AutoPBSC procedure. In comprission with MNC procedure of COBE Spectra cell seperator, the CD34 count collected by MNC procedure of Spectra Optia Seperator did not show significant difference, but the CD34 cell count collected by MNC procedure of Spectra Optia was higher than that collected by MNC procedure of COBE Spectra cell separator (P<0.05). The platelet count and hemoglobin level collected by MNC procedure of Spectra Optia were lower than those before collection. The adverse reactions in the 3 procedures were similar, and the patients could tolerate them.
The AutoPBSC procedure of COBE Spectra and MNC procedure of Spectra Optia are better than MNC procedure of COBE Spectra for autologous peripheral blood hematopoietic stem cells collection. The loss of blood platelet and hemoglobin after collection is lowest in MNC procedure of Spectra Optia.
观察使用COBE Spectra细胞分离仪的自体外周血干细胞采集程序（autoPBSC程序）和单核细胞采集程序（MNC程序）以及Spectra Optia细胞分离仪的MNC程序进行自体外周血干细胞采集时的疗效及不良反应。
采用COBE Spectra血细胞分离仪的autoPBSC程序和MNC程序以及Spectra Optia血细胞分离仪的MNC程序，采集41例患者的自体外周血造血干细胞。观察3种采集程序所采集的单核细胞（MNC）和CD34细胞数量、血红蛋白（Hb）下降及血小板减少情况以及患者的不良反应。
3组全血处理及采集时间基本相同时，COBE Spectra和Spectra Optia的MNC程序所采集的MNC计数高于COBE Spectra的autoPBSC程序，但CD34细胞计数低于autoPBSC程序所采集的（P<0.05）。COBE Spectra和Spectra Optia的MNC程序最终采集产品体积大于autoPBSC程序。与COBE Spectra细胞分离仪的MNC程序相比，Spectra Optia分离仪的MNC程序所采集的CD34计数差异无统计学意义，但Spectra Optia的MNC程序所采集的CD34细胞计数高于COBE Spectra细胞分离仪的MNC程序（P<0.05）。Spectra Optia的MNC程序采集后血小板计数和血红蛋白水平低于采集前。3种程序的不良反应相似，患者均可耐受。
对于自体外周血造血干细胞采集，COBE Spectra的autoPBSC程序和Spectra Optia的MNC程序优于COBE Spectra的MNC程序。Spectra Optia的MNC程序采集后血小板和血红蛋白损失最少。
