Hiruma K, Nakayama S, Okuyama Y
Transfusion Service, Tokyo Metropolitan Komagome Hospital, Japan.
Ther Apher. 1998 Nov;2(4):273-6. doi: 10.1111/j.1744-9987.1998.tb00121.x.
Recently, a completely automated procedure, the AutoPBSC program for the COBE Spectra cell separator, has been developed for peripheral blood stem cell harvest (PBSCH). We compared the performance of the AutoPBSC program with the standard mononuclear cell (MNC) program in the same patients and in a donor. Peripheral blood stem cells (PBSC) were collected from 3 patients or a donor alternately using the MNC program and the AutoPBSC program in a course of PBSC mobilization. Equal blood volume was processed from each patient (200 ml/kg) and a donor (150 ml/kg). We used a harvest volume of 3 ml and a chase volume of 7 ml in all AutoPBSC procedures. The procedure duration was almost equivalent for both programs. The volume of products was significantly lower in the AutoPBSC program (71 +/- 13 ml) than in the MNC program (183 +/- 30 ml). MNC yields were fewer, and total nucleated cell (TNC) and MNC collection efficiency was less for the AutoPBSC program compared to the MNC program. The CD34+ cell collection efficiency was less for the AutoPBSC program (26.5 +/- 13.7%, compared with 77.7 +/- 60.6%; p > 0.05). The contamination of platelets and red cells was significantly less in the AutoPBSC program than in the MNC program. In conclusion, we consider that the collection efficiency in the new program should be improved by modification of parameters because there exist great advantages to automated procedures.
最近，已经开发出一种完全自动化的程序，即用于COBE Spectra细胞分离器的AutoPBSC程序，用于外周血干细胞采集（PBSCH）。我们在同一患者和一名供体中比较了AutoPBSC程序与标准单核细胞（MNC）程序的性能。在PBSC动员过程中，交替使用MNC程序和AutoPBSC程序从3名患者或一名供体中采集外周血干细胞（PBSC）。每位患者（200 ml/kg）和供体（150 ml/kg）处理相同体积的血液。在所有AutoPBSC程序中，我们使用3 ml的采集体积和7 ml的追赶体积。两个程序的操作持续时间几乎相同。AutoPBSC程序的产品体积（71±13 ml）明显低于MNC程序（183±30 ml）。与MNC程序相比，AutoPBSC程序的MNC产量较少，总核细胞（TNC）和MNC采集效率较低。AutoPBSC程序的CD34+细胞采集效率较低（26.5±13.7%，而MNC程序为77.7±60.6%；p>0.05）。AutoPBSC程序中血小板和红细胞的污染明显低于MNC程序。总之，我们认为新程序的采集效率应通过参数修改来提高，因为自动化程序有很大优势。
