Davies C R, Jones L D, Nuttall P A
NERC Institute of Virology, Oxford, U.K.
J Gen Virol. 1989 Sep;70 ( Pt 9):2461-8. doi: 10.1099/0022-1317-70-9-2461.
Interference between arboviruses in a naturally infected tick vector is reported for the first time. Rhipicephalus appendiculatus nymphs were dually infected with Thogoto (THO) virus, a tick-borne virus, similar to members of the family Orthomyxoviridae. In the first series of experiments examining 'inter-stadial' interference, larvae were orally infected with a temperature-sensitive (ts) mutant, and after moulting the nymphs were superinfected with the wild-type (wt) virus. In the second series of experiments examining 'intra-stadial' interference, nymphs were dually infected by interrupted feeding; the time interval between infective feeds was either shorter than 24 h or lasted for 10 days. Interference was demonstrated by the inability of wt virus to replicate in ticks previously infected with ts virus. Both inter- and intra-stadial interference were observed and complete interference was detected in 78% of dually infected nymphs. A pool of dually infected ticks, in which intra-stadial interference had been detected, failed to transmit the superinfecting virus after moulting.
首次报道了在自然感染的蜱传播媒介中虫媒病毒之间的干扰现象。微小牛蜱若虫被与正粘病毒科成员相似的蜱传病毒托戈托（THO）病毒双重感染。在第一组检测“不同龄期间”干扰的实验中，幼虫经口感染一种温度敏感（ts）突变体，蜕皮后的若虫再用野生型（wt）病毒进行超感染。在第二组检测“同龄期内”干扰的实验中，若虫通过间断喂食进行双重感染；感染性喂食之间的时间间隔要么短于24小时，要么持续10天。wt病毒无法在先前感染ts病毒的蜱中复制，证明了干扰现象的存在。同龄期内和不同龄期间的干扰均被观察到，并且在78%的双重感染若虫中检测到完全干扰。一组检测到同龄期内干扰的双重感染蜱，在蜕皮后未能传播超感染病毒。
