Lugar D W, Rhoads M L, Clark-Deener S G, Callahan S R, Revercomb A K, Prusa K J, Estienne M J
1Department of Animal and Poultry Sciences,Virginia Tech,Blacksburg,VA 24061,USA.
2Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine,Blacksburg,VA 24061,USA.
Animal. 2017 Apr;11(4):643-649. doi: 10.1017/S1751731116002081. Epub 2016 Oct 27.
The objective was to determine the effects of immunization against gonadotropin-releasing hormone on reproductive characteristics in boars. A total of 72 boars were used in a randomized design with three treatments: single immunization (SI) (10 weeks of age) or double immunization (DI) (10 and 15 weeks of age) with Improvest® and intact controls (no Improvest®; CNT) (n=24/group). At 10, 15, 20, 25 and 40 weeks of age, blood was collected and serum harvested to evaluate testosterone concentrations. Testosterone concentrations were less for DI boars compared with CNT boars and SI boars at 20 and 25 weeks (P<0.001), but not at 40 weeks of age. At week 25, 18 pigs (n=6/group) were sacrificed and testes were removed, weighed and measured, and seminiferous tubules were examined and scored using histological slides of testes parenchyma. A sample of neck fat was assessed for boar taint aroma. All testicular measurements and weights and seminiferous tubule scores were less for DI boars compared with SI and CNT boars (P<0.001). More (P<0.05) SI and CNT boars had detectable boar taint aroma than DI boars. Libido was assessed at 32, 36, 47, 60 and 63 weeks of age and semen collected at 60 weeks of age was analyzed for indicators of quality. There were no effects of treatment (P=0.41) or treatment by week (P=0.71) on libido. Semen volume, gel weight and total number of sperm cells, determined in a subset of boars (n=3/treatment), were not different among treatments. Sperm concentration was greater for DI than SI (P=0.01), and tended to be greater for DI compared with CNT (P=0.10). Sperm motility tended to be greater for DI boars compared with CNT boars (P=0.066). In conclusion, our results show that there are no long-term effects of immunocastration on reproductive characteristics in boars.
目的是确定针对促性腺激素释放激素进行免疫对公猪生殖特性的影响。总共72头公猪用于一项随机设计，有三种处理方式：单次免疫（SI）（10周龄）或两次免疫（DI）（10周龄和15周龄），使用Improvest®，以及完整对照（未使用Improvest®；CNT）（每组n = 24头）。在10、15、20、25和40周龄时采集血液并收获血清，以评估睾酮浓度。在20周和25周时，DI组公猪的睾酮浓度低于CNT组公猪和SI组公猪（P < 0.001），但在40周龄时并非如此。在25周时，处死18头猪（每组n = 6头），取出睾丸，称重并测量，使用睾丸实质组织切片对生精小管进行检查和评分。对颈部脂肪样本进行公猪异味香气评估。与SI组和CNT组公猪相比，DI组公猪的所有睾丸测量值、重量和生精小管评分均较低（P < 0.001）。与DI组公猪相比，有更多（P < 0.05）的SI组和CNT组公猪可检测到公猪异味香气。在32、36、47、60和63周龄时评估性欲，并对60周龄时采集的精液进行质量指标分析。处理方式（P = 0.41）或处理方式与周龄的交互作用（P = 0.71）对性欲均无影响。在一部分公猪（每种处理n = 3头）中测定的精液体积、凝胶重量和精子细胞总数在各处理之间没有差异。DI组的精子浓度高于SI组（P = 0.01），并且与CNT组相比，DI组的精子浓度有升高趋势（P = 0.10）。与CNT组公猪相比，DI组公猪的精子活力有升高趋势（P = 0.066）。总之，我们的结果表明，免疫去势对公猪的生殖特性没有长期影响。
