Picazo-Bueno José Ángel, Trusiak Maciej, García Javier, Patorski Krzysztof, Micó Vicente
Opt Lett. 2018 Mar 1;43(5):1007-1010. doi: 10.1364/OL.43.001007.
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46 NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic, samples showing perfect agreement with the results retrieved from a temporal phase-shifting algorithm.
希尔伯特 - 黄单次空间复用干涉显微镜（H2S2MIM）是一种稳健、快速且准确的单次相位估计算法的实现方式，它以极其简单、低成本且高度稳定的方法，通过部分相干照明将明场显微镜转换为全息显微镜。总体而言，H2S2MIM为商用非全息显微镜增添了高速（视频帧率）定量相位成像能力，并改进了相位重建（降低相干噪声）。该技术已在常规的奥林巴斯BX - 60正置显微镜中使用20×/0.46 NA物镜进行了验证，适用于静态和动态样本，结果与从时间相位偏移算法获得的结果完全一致。
