a SCIENT-Research Center for Instrumental Analysis , Tancabesti-Snagov , Romania.
b Department of Analytical Chemistry and Toxicology , Hanoi University of Pharmacy , Hanoi , Vietnam.
Crit Rev Anal Chem. 2017 May 4;47(3):194-203. doi: 10.1080/10408347.2016.1253454. Epub 2016 Oct 27.
Vibrational spectroscopy (Infrared (IR) and Raman) and, in particular, micro-spectroscopy and micro-spectroscopic imaging have been used to characterize developmental changes in tissues, to monitor these changes in cell cultures and to detect disease and drug-induced modifications. The conventional methods for biochemical and histophatological tissue characterization necessitate complex and "time-consuming" sample manipulations and the results are rarely quantifiable. The spectroscopy of molecular vibrations using mid-IR or Raman techniques has been applied to samples of human tissue. This article reviews the application of these vibrational spectroscopic techniques for analysis of biological tissue published between 2005 and 2015.
振动光谱（红外（IR）和拉曼），特别是微光谱和微光谱成像，已被用于描述组织的发育变化，监测细胞培养物中的这些变化，并检测疾病和药物诱导的变化。用于生化和组织病理学组织特征描述的常规方法需要复杂和“耗时”的样本处理，并且结果很少是定量的。使用中红外或拉曼技术对分子振动的光谱进行了研究。本文回顾了 2005 年至 2015 年间发表的关于这些振动光谱技术在生物组织分析中的应用的文章。
