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真核细胞周期中铁的核内掺入。

Nuclear incorporation of iron during the eukaryotic cell cycle.

作者信息

Robinson Ian, Yang Yang, Zhang Fucai, Lynch Christophe, Yusuf Mohammed, Cloetens Peter

机构信息

Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxon OX11 0FA, UK.

ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.

出版信息

J Synchrotron Radiat. 2016 Nov 1;23(Pt 6):1490-1497. doi: 10.1107/S1600577516012807. Epub 2016 Oct 18.

DOI:10.1107/S1600577516012807
PMID:27787255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5082466/
Abstract

Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.

摘要

扫描X射线荧光显微镜已被用于探测细胞核内硫、磷和铁的分布。发现可能起源于细胞周期不同阶段的细胞核显示出非常不同的铁含量水平，且分布极不均匀。磷和硫信号可能来自DNA及相关核小体，在所有细胞核中含量较高且相对均匀；这些与相同样品的X射线相衬投影显微镜图像相符。文中讨论了铁掺入的可能原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/8a4ce2807fcf/s-23-01490-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/c1990b553d2b/s-23-01490-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/c3156e110f3f/s-23-01490-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/575d1bd08060/s-23-01490-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/2891e8be1384/s-23-01490-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/e8072d251865/s-23-01490-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/9a30cc0dfad5/s-23-01490-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/8a4ce2807fcf/s-23-01490-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/c1990b553d2b/s-23-01490-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/c3156e110f3f/s-23-01490-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/575d1bd08060/s-23-01490-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/2891e8be1384/s-23-01490-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/e8072d251865/s-23-01490-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/9a30cc0dfad5/s-23-01490-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb70/5082466/8a4ce2807fcf/s-23-01490-fig7.jpg

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