European Synchrotron Radiation Facility, Grenoble, France.
J Struct Biol. 2012 Feb;177(2):239-47. doi: 10.1016/j.jsb.2011.12.005. Epub 2011 Dec 11.
Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
硬 X 射线荧光显微镜和放大相衬成像相结合，可获得整个细胞中金属浓度的定量映射。该实验是在欧洲同步辐射设施（ESRF）的纳米成像站 ID22NI 上对冻干细胞进行的。X 射线荧光分析给出了大多数主要、次要和微量元素的面质量；使用已知成分的生物标准进行了验证。定量相衬成像提供了投影质量的映射，并使用校准样品以及与原子力显微镜和扫描传输离子显微镜的比较进行了验证。到目前为止，使用 X 射线荧光显微镜在亚细胞水平上进行绝对定量是不可能的，但可以使用所提出的方法来实现。
