Sawa Teiji, Hamaoka Saeko, Kinoshita Mao, Kainuma Atsushi, Naito Yoshifumi, Akiyama Koichi, Kato Hideya
Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Toxins (Basel). 2016 Oct 26;8(11):307. doi: 10.3390/toxins8110307.
ExoU, a type III secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. Through use of a recently updated bacterial genome database, protein sequences predicted to be homologous to ExoU were identified in 17 other species (, , , , , , , , , , , , , , and ) and 8 Gram-negative bacteria from three other genera (, , and ). In the alignment of the predicted primary amino acid sequences used for the phylogenetic analyses, both highly conserved and nonconserved parts of the toxin were discovered among the various species. Further comparative studies of the predicted ExoU homologs should provide us with more detailed information about the unique characteristics of the ExoU toxin.
ExoU是一种具有类Patatin磷脂酶活性的III型分泌毒素和主要毒力因子，可导致免疫功能低下患者发生急性肺损伤和败血症。通过使用最近更新的细菌基因组数据库，在其他17个物种（、、、、、、、、、、、、、、和）以及来自其他三个属（、和）的8种革兰氏阴性细菌中鉴定出了预测与ExoU同源的蛋白质序列。在用于系统发育分析的预测一级氨基酸序列比对中，发现该毒素在不同物种中既有高度保守的部分，也有非保守的部分。对预测的ExoU同源物进行进一步的比较研究，应该能为我们提供有关ExoU毒素独特特征的更详细信息。
