Ly Nguyễn Hoàng, Seo Chulhun, Joo Sang-Woo
Department of Chemistry, Soongsil University, Seoul 156-743, Korea.
Department of Information Communication, Materials, and Chemistry Convergence Technology, Soongsil University, Seoul 156-743, Korea.
Sensors (Basel). 2016 Oct 26;16(11):1785. doi: 10.3390/s16111785.
A facile, selective, and sensitive detection method for the Cu ions in environmental and biological solutions has been newly developed by observing the unique CN stretching peaks at ~2108 cm upon the dissociative adsorption of glycine (GLY) in hydrazine buffer on gold nanoparticles (AuNPs). The relative abundance of Cu species on AuNPs was identified from X-ray photoelectron spectroscopy analysis. UV-Vis spectra also indicated that the Au particles aggregated to result in the color change owing to the destabilization induced by the GLY-Cu complex. The CN stretching band at ~2108 cm could be observed to indicate the formation of the CN species from GLY on the hydrazine-covered AuNP surfaces. The other ions of Fe, Fe, Hg, Mg, Mn, Ni, Zn, Cr, Co, Cd, Pb, Ca, NH₄⁺, Na⁺, and K⁺ at high concentrations of 50 µM did not produce such spectral changes. The detection limit based on the CN band for the determination of the Cu ion could be estimated to be as low as 500 nM in distilled water and 1 µM in river water, respectively. We attempted to apply our method to estimate intracellular ion detection in cancer cells for more practical purposes.
通过观察甘氨酸（GLY）在肼缓冲液中于金纳米颗粒（AuNPs）上的解离吸附时在2108 cm处独特的CN伸缩峰，新开发了一种用于环境和生物溶液中铜离子的简便、选择性和灵敏的检测方法。通过X射线光电子能谱分析确定了AuNPs上铜物种的相对丰度。紫外-可见光谱还表明，由于GLY-Cu络合物引起的去稳定作用，金颗粒聚集导致颜色变化。可以观察到在2108 cm处的CN伸缩带，表明在肼覆盖的AuNP表面上由GLY形成了CN物种。50 µM高浓度的其他离子Fe、Fe、Hg、Mg、Mn、Ni、Zn、Cr、Co、Cd、Pb、Ca、NH₄⁺、Na⁺和K⁺不会产生这种光谱变化。基于CN带测定铜离子的检测限在蒸馏水中估计低至500 nM，在河水中为1 µM。为了更实际的目的，我们尝试应用我们的方法来估计癌细胞中的细胞内离子检测。
