• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

减数分裂特异性黏连蛋白复合体在哺乳动物精子发生中的不同作用。

Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis.

作者信息

Biswas Uddipta, Hempel Kai, Llano Elena, Pendas Alberto, Jessberger Rolf

机构信息

Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Centro de Investigacion del Cancer (CSIC-USAL), Campus Miguel de Unamuno, Salamanca, Spain.

出版信息

PLoS Genet. 2016 Oct 28;12(10):e1006389. doi: 10.1371/journal.pgen.1006389. eCollection 2016 Oct.

DOI:10.1371/journal.pgen.1006389
PMID:27792785
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5085059/
Abstract

Mammalian meiocytes feature four meiosis-specific cohesin proteins in addition to ubiquitous ones, but the roles of the individual cohesin complexes are incompletely understood. To decipher the functions of the two meiosis-specific kleisins, REC8 or RAD21L, together with the only meiosis-specific SMC protein SMC1β, we generated Smc1β-/-Rec8-/- and Smc1β-/-Rad21L-/- mouse mutants. Analysis of spermatocyte chromosomes revealed that besides SMC1β complexes, SMC1α/RAD21 and to a small extent SMC1α/REC8 contribute to chromosome axis length. Removal of SMC1β and RAD21L almost completely abolishes all chromosome axes. The sex chromosomes do not pair in single or double mutants, and autosomal synapsis is impaired in all mutants. Super resolution microscopy revealed synapsis-associated SYCP1 aberrantly deposited between sister chromatids and on single chromatids in Smc1β-/-Rad21L-/- cells. All mutants show telomere length reduction and structural disruptions, while wild-type telomeres feature a circular TRF2 structure reminiscent of t-loops. There is no loss of centromeric cohesion in both double mutants at leptonema/early zygonema, indicating that, at least in the mutant backgrounds, an SMC1α/RAD21 complex provides centromeric cohesion at this early stage. Thus, in early prophase I the most prominent roles of the meiosis-specific cohesins are in axis-related features such as axis length, synapsis and telomere integrity rather than centromeric cohesion.

摘要

除了普遍存在的黏连蛋白外，哺乳动物的减数分裂细胞还具有四种减数分裂特异性黏连蛋白，但各个黏连蛋白复合体的作用尚未完全明确。为了解析两种减数分裂特异性kleisin蛋白REC8或RAD21L以及唯一的减数分裂特异性SMC蛋白SMC1β的功能，我们构建了Smc1β-/-Rec8-/-和Smc1β-/-Rad21L-/-小鼠突变体。对精母细胞染色体的分析表明，除了SMC1β复合体，SMC1α/RAD21以及在较小程度上的SMC1α/REC8也对染色体轴长度有贡献。去除SMC1β和RAD21L几乎完全消除了所有染色体轴。性染色体在单突变体或双突变体中都不配对，并且所有突变体中的常染色体联会均受损。超分辨率显微镜显示，在Smc1β-/-Rad21L-/-细胞中，与联会相关的SYCP1异常沉积在姐妹染色单体之间和单个染色单体上。所有突变体均表现出端粒长度缩短和结构破坏，而野生型端粒具有类似于t环的环状TRF2结构。在细线期/早偶线期，两个双突变体中着丝粒黏连均未丧失，这表明至少在突变背景下，SMC1α/RAD21复合体在这一早期阶段提供着丝粒黏连。因此，在减数分裂前期I早期，减数分裂特异性黏连蛋白最显著的作用在于与轴相关的特征，如轴长度、联会和端粒完整性，而非着丝粒黏连。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/c6ea87b3dde4/pgen.1006389.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/5b795ae00d24/pgen.1006389.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/59a5c79840fb/pgen.1006389.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/0464d5203812/pgen.1006389.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/a8371b255a48/pgen.1006389.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/e3f2bd805b4d/pgen.1006389.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/180afc232a80/pgen.1006389.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/45af6c363f74/pgen.1006389.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/c6ea87b3dde4/pgen.1006389.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/5b795ae00d24/pgen.1006389.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/59a5c79840fb/pgen.1006389.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/0464d5203812/pgen.1006389.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/a8371b255a48/pgen.1006389.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/e3f2bd805b4d/pgen.1006389.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/180afc232a80/pgen.1006389.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/45af6c363f74/pgen.1006389.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a6/5085059/c6ea87b3dde4/pgen.1006389.g008.jpg

相似文献

1
Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis.减数分裂特异性黏连蛋白复合体在哺乳动物精子发生中的不同作用。
PLoS Genet. 2016 Oct 28;12(10):e1006389. doi: 10.1371/journal.pgen.1006389. eCollection 2016 Oct.
2
Genetic Interactions Between the Meiosis-Specific Cohesin Components, STAG3, REC8, and RAD21L.减数分裂特异性黏连蛋白组分STAG3、REC8和RAD21L之间的遗传相互作用
G3 (Bethesda). 2016 Jun 1;6(6):1713-24. doi: 10.1534/g3.116.029462.
3
Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes.减数分裂特异性黏连蛋白组分Stag3对于维持着丝粒染色单体黏连至关重要，并且是DNA修复以及同源染色体之间联会所必需的。
PLoS Genet. 2014 Jul 3;10(7):e1004413. doi: 10.1371/journal.pgen.1004413. eCollection 2014 Jul.
4
Meiotic cohesin SMC1β provides prophase I centromeric cohesion and is required for multiple synapsis-associated functions.减数分裂黏合蛋白 SMC1β 提供前期 I 着丝粒黏合，并且是多个联会相关功能所必需的。
PLoS Genet. 2013;9(12):e1003985. doi: 10.1371/journal.pgen.1003985. Epub 2013 Dec 26.
5
Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis.在哺乳动物减数分裂过程中，Rec8蛋白在减数分裂染色体上出现时空选择性缺失。
J Cell Sci. 2003 Jul 1;116(Pt 13):2781-90. doi: 10.1242/jcs.00495. Epub 2003 May 20.
6
STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis.STAG3 介导的 REC8 黏合蛋白复合物稳定促进减数分裂过程中的染色体联会。
EMBO J. 2014 Jun 2;33(11):1243-55. doi: 10.1002/embj.201387329. Epub 2014 May 5.
7
Meiotic cohesin STAG3 is required for chromosome axis formation and sister chromatid cohesion.减数分裂黏连蛋白 STAG3 对于染色体轴的形成和姐妹染色单体的黏合是必需的。
EMBO J. 2014 Jun 2;33(11):1256-70. doi: 10.1002/embj.201387330. Epub 2014 May 5.
8
High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation.REC8的高浓度会限制姐妹染色单体轴，并阻止异常联会复合体的形成。
EMBO Rep. 2016 Jun;17(6):901-13. doi: 10.15252/embr.201642030. Epub 2016 May 11.
9
Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1beta and SMC3.减数分裂黏连蛋白REC8在黏连蛋白SMC1β和SMC3之前标记大鼠联会复合体的轴元件。
J Cell Biol. 2003 Mar 3;160(5):657-70. doi: 10.1083/jcb.200212080.
10
Changes in the expression and localization of cohesin subunits during meiosis in a non-mammalian vertebrate, the medaka fish.非哺乳类脊椎动物青鳉鱼减数分裂过程中黏连蛋白亚基的表达和定位变化。
Gene Expr Patterns. 2004 Sep;4(5):495-504. doi: 10.1016/j.modgep.2004.03.004.

引用本文的文献

1
Testis-Specific PDHA2 Is Required for Proper Meiotic Recombination and Chromosome Organisation During Spermatogenesis.睾丸特异性PDHA2是精子发生过程中正确减数分裂重组和染色体组织所必需的。
Cell Prolif. 2025 Jul;58(7):e70003. doi: 10.1111/cpr.70003. Epub 2025 Feb 20.
2
Essential Regulation of YAP1 in Fate Determinations of Spermatogonial Stem Cells and Male Fertility by Interacting with RAD21 and Targeting NEDD4 in Humans and Mice.YAP1通过与RAD21相互作用并靶向人类和小鼠中的NEDD4对精原干细胞命运决定和雄性生育能力的关键调控
Research (Wash D C). 2024 Dec 10;7:0544. doi: 10.34133/research.0544. eCollection 2024.
3

本文引用的文献

1
Genetic Interactions Between the Meiosis-Specific Cohesin Components, STAG3, REC8, and RAD21L.减数分裂特异性黏连蛋白组分STAG3、REC8和RAD21L之间的遗传相互作用
G3 (Bethesda). 2016 Jun 1;6(6):1713-24. doi: 10.1534/g3.116.029462.
2
MAJIN Links Telomeric DNA to the Nuclear Membrane by Exchanging Telomere Cap.MAJIN 通过交换端粒帽将端粒 DNA 与核膜联系起来。
Cell. 2015 Nov 19;163(5):1252-1266. doi: 10.1016/j.cell.2015.10.030. Epub 2015 Nov 5.
3
Recombination, Pairing, and Synapsis of Homologs during Meiosis.减数分裂过程中同源染色体的重组、配对和联会
Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization.
温度调节负超螺旋以调节减数分裂交叉和染色体组织。
Sci China Life Sci. 2024 Nov;67(11):2426-2443. doi: 10.1007/s11427-024-2671-1. Epub 2024 Jul 23.
4
SCC3 is an axial element essential for homologous chromosome pairing and synapsis.SCC3 是同源染色体配对和联会所必需的轴向元件。
Elife. 2024 Jun 12;13:RP94180. doi: 10.7554/eLife.94180.
5
Towards a "Testis in a Dish": Generation of Mouse Testicular Organoids that Recapitulate Testis Structure and Expression Profiles.迈向“类睾丸”：生成可再现睾丸结构和表达谱的小鼠睾丸类器官。
Int J Biol Sci. 2024 Jan 12;20(3):1024-1041. doi: 10.7150/ijbs.89480. eCollection 2024.
6
METTL3/IGF2BP2 axis affects the progression of colorectal cancer by regulating m6A modification of STAG3.METTL3/IGF2BP2 轴通过调节 STAG3 的 m6A 修饰影响结直肠癌的进展。
Sci Rep. 2023 Oct 12;13(1):17292. doi: 10.1038/s41598-023-44379-x.
7
Synaptonemal Complex in Human Biology and Disease.人类生物学与疾病中的联会复合体
Cells. 2023 Jun 25;12(13):1718. doi: 10.3390/cells12131718.
8
Cohesin SMC1β promotes closed chromatin and controls TERRA expression at spermatocyte telomeres.黏连蛋白 SMC1β 促进减数分裂前期精母细胞端粒的闭和染色质形成，并控制端粒相关 RNA（TERRA）的表达。
Life Sci Alliance. 2023 May 9;6(7). doi: 10.26508/lsa.202201798. Print 2023 Jul.
9
RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits.RBM46 对于配子发生是必不可少的，并在影响减数分裂黏连蛋白亚基的转录后作用中发挥功能。
Protein Cell. 2022 Sep 14;14(1):51-63. doi: 10.1093/procel/pwac040. eCollection 2023 Jan.
10
Chromosome architecture and homologous recombination in meiosis.减数分裂中的染色体结构与同源重组
Front Cell Dev Biol. 2023 Jan 6;10:1097446. doi: 10.3389/fcell.2022.1097446. eCollection 2022.
Cold Spring Harb Perspect Biol. 2015 May 18;7(6):a016626. doi: 10.1101/cshperspect.a016626.
4
Complex elaboration: making sense of meiotic cohesin dynamics.复杂阐述：理解减数分裂黏连蛋白动力学
FEBS J. 2015 Jul;282(13):2426-43. doi: 10.1111/febs.13301. Epub 2015 May 9.
5
Structural and functional adaptations of the mammalian nuclear envelope to meet the meiotic requirements.哺乳动物核膜的结构和功能适应性以满足减数分裂的需求。
Nucleus. 2015;6(2):93-101. doi: 10.1080/19491034.2015.1004941. Epub 2015 Feb 12.
6
Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes.减数分裂特异性黏连蛋白组分Stag3对于维持着丝粒染色单体黏连至关重要，并且是DNA修复以及同源染色体之间联会所必需的。
PLoS Genet. 2014 Jul 3;10(7):e1004413. doi: 10.1371/journal.pgen.1004413. eCollection 2014 Jul.
7
STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis.STAG3 介导的 REC8 黏合蛋白复合物稳定促进减数分裂过程中的染色体联会。
EMBO J. 2014 Jun 2;33(11):1243-55. doi: 10.1002/embj.201387329. Epub 2014 May 5.
8
Meiotic cohesin STAG3 is required for chromosome axis formation and sister chromatid cohesion.减数分裂黏连蛋白 STAG3 对于染色体轴的形成和姐妹染色单体的黏合是必需的。
EMBO J. 2014 Jun 2;33(11):1256-70. doi: 10.1002/embj.201387330. Epub 2014 May 5.
9
STAG3 is a strong candidate gene for male infertility.STAG3是男性不育的一个强有力的候选基因。
Hum Mol Genet. 2014 Jul 1;23(13):3421-31. doi: 10.1093/hmg/ddu051. Epub 2014 Mar 7.
10
Meiotic cohesin SMC1β provides prophase I centromeric cohesion and is required for multiple synapsis-associated functions.减数分裂黏合蛋白 SMC1β 提供前期 I 着丝粒黏合，并且是多个联会相关功能所必需的。
PLoS Genet. 2013;9(12):e1003985. doi: 10.1371/journal.pgen.1003985. Epub 2013 Dec 26.