Park Yong Il, Kim Eunha, Huang Chen-Han, Park Ki Soo, Castro Cesar M, Lee Hakho, Weissleder Ralph
Department of Systems Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Bioconjug Chem. 2017 Jan 18;28(1):33-37. doi: 10.1021/acs.bioconjchem.6b00524. Epub 2016 Nov 7.
The use of inorganic nanoparticles (NPs) for biosensing requires that they exhibit high colloidal stability under various physiological conditions. Here, we report on a general approach to render hydrophobic NPs into hydrophilic ones that are ready for bioconjugation. The method uses peglyated polymers conjugated with multiple dopamines, which results in multidentate coordination. As proof-of-concept, we applied the coating to stabilize ferrite and lanthanide NPs synthesized by thermal decomposition. Both polymer-coated NPs showed excellent water solubility and were stable at high salt concentrations under physiological conditions. We used these NPs as molecular-sensing agents to detect exosomes and bacterial nucleic acids.
将无机纳米颗粒（NPs）用于生物传感要求它们在各种生理条件下都具有高胶体稳定性。在此，我们报道了一种将疏水性NPs转变为亲水性NPs以用于生物共轭的通用方法。该方法使用与多个多巴胺共轭的聚乙二醇化聚合物，这会导致多齿配位。作为概念验证，我们应用该涂层来稳定通过热分解合成的铁氧体和镧系元素NPs。两种聚合物包覆的NPs均表现出优异的水溶性，并且在生理条件下的高盐浓度下稳定。我们使用这些NPs作为分子传感剂来检测外泌体和细菌核酸。
