隐形眼镜护理液中自由生活原生动物的种类

Repertoire of free-living protozoa in contact lens solutions.

作者信息

Bouchoucha Ibtissem, Aziz Aurore, Hoffart Louis, Drancourt Michel

机构信息

Pôle des Maladies Infectieuses, Hôpital de la Timone, Marseille, France.

Ophthalmology Department, Hôpital de la Timone, Marseille, France.

出版信息

BMC Ophthalmol. 2016 Oct 29;16(1):191. doi: 10.1186/s12886-016-0370-6.

DOI:10.1186/s12886-016-0370-6

PMID:27793130

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5086047/

Abstract

BACKGROUND

The repertoire of free-living protozoa in contact lens solutions is poorly known despite the fact that such protozoa may act as direct pathogens and may harbor intra-cellular pathogens.

METHODS

Between 2009 and 2014, the contact lens solutions collected from patients presenting at our Ophthalmology Department for clinically suspected keratitis, were cultured on non-nutrient agar examined by microscope for the presence of free-living protozoa. All protozoa were identified by 18S rRNA gene sequencing.

RESULTS

A total of 20 of 233 (8.6 %) contact lens solution specimens collected from 16 patients were cultured. Acanthamoeba amoeba in 16 solutions (80 %) collected from 12 patients and Colpoda steini, Cercozoa sp., Protostelium sp. and a eukaryotic more closely related to Vermamoeba sp., were each isolated in one solution. Cercozoa sp., Colpoda sp., Protostelium sp. and Vermamoeba sp. are reported for the first time as contaminating contact lens solutions.

CONCLUSION

The repertoire of protozoa in contact lens solutions is larger than previously known.

摘要

背景

尽管自由生活的原生动物可能作为直接病原体并携带细胞内病原体，但我们对隐形眼镜护理液中的此类原生动物种类却知之甚少。

方法

在2009年至2014年期间，从我院眼科临床疑似角膜炎患者处收集隐形眼镜护理液，接种于不含营养成分的琼脂培养基上培养，通过显微镜检查是否存在自由生活的原生动物。所有原生动物均通过18S rRNA基因测序进行鉴定。

结果

从16例患者中收集的233份隐形眼镜护理液样本中，共有20份（8.6%）培养出微生物。从12例患者的16份护理液样本（80%）中培养出棘阿米巴原虫，在另外一份护理液样本中分别分离出施氏肾形虫、Cercozoa属、原柄菌属和一种与维氏阿米巴属关系更密切的真核生物。Cercozoa属、肾形虫属、原柄菌属和维氏阿米巴属作为隐形眼镜护理液污染物首次被报道。