嗜热四膜虫温度敏感突变体的大规模分离与育性测试
Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.
作者信息
Bruns P J, Sanford Y M
出版信息
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3355-8. doi: 10.1073/pnas.75.7.3355.
Abstract
A set of 239 heat-sensitive (38 degrees) and 16 cold-sensitive (18 degrees) conditional mutants of Tetrahymena has been generated by combining techniques to manipulate large numbers of clones with a method for the selection of self-fertilized cells after mutagen treatment. A simple technique is presented for determining the fertility of individual clones; 179 of the clones in this set (71%) are fertile. The fertile conditional mutants have already been shown to have lesions in a number of diverse functions, including nucleic acid metabolism, mobility, cell cycle, and cortical pattern.
摘要
通过将大量克隆操作技术与诱变处理后自交细胞的选择方法相结合，已产生了一组239个嗜热（38摄氏度）和16个嗜冷（18摄氏度）的嗜热栖热菌条件突变体。本文介绍了一种确定单个克隆育性的简单技术；该组中的179个克隆（71%）是可育的。这些可育的条件突变体已被证明在许多不同功能中存在缺陷，包括核酸代谢、运动性、细胞周期和皮层模式。
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