中国西北部新疆地区奶牛犊牛中微小隐孢子虫J基因型的优势地位。
Dominance of Enterocytozoon bieneusi genotype J in dairy calves in Xinjiang, Northwest China.
作者信息
Qi Meng, Jing Bo, Jian Fuchun, Wang Rongjun, Zhang Sumei, Wang Haiyan, Ning Changshen, Zhang Longxian
机构信息
College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China; College of Animal Science, Tarim University, Tarim Road 1487, Alar, Xinjiang 843300, PR China.
College of Animal Science, Tarim University, Tarim Road 1487, Alar, Xinjiang 843300, PR China.
出版信息
Parasitol Int. 2017 Feb;66(1):960-963. doi: 10.1016/j.parint.2016.10.019. Epub 2016 Oct 26.
Enterocytozoon bieneusi is the most common microsporidia species in humans and has a variety of animal hosts. To assess the prevalence and molecular characteristics of E. bieneusi in dairy calves in the Xinjiang Uyghur Autonomous Region of China, 514 fecal samples were collected from 15 farms and examined by polymerase chain reaction based on the internal transcribed spacer of the ribosomal RNA gene of E. bieneusi. The overall prevalence of E. bieneusi in calves was 16.5% (85/514). No significant difference in prevalence was observed between pre- and post-weaned calves. Sequence analysis of ITS nucleotide sequences identified six known genotypes (BEB4, CC4, D, I, J, and EbpC), five of these previously detected in humans. Genotype J was the most prevalent genotype (57/85) and was identified on 11 farms. The high prevalence of zoonotic E. bieneusi genotypes in dairy calves suggests they are a potential source of zoonotic infection in humans.
肠道微孢子虫是人类中最常见的微孢子虫物种，并且有多种动物宿主。为评估中国新疆维吾尔自治区奶牛犊中肠道微孢子虫的流行情况和分子特征，从15个养殖场收集了514份粪便样本，并基于肠道微孢子虫核糖体RNA基因的内转录间隔区，通过聚合酶链反应进行检测。犊牛中肠道微孢子虫的总体流行率为16.5%（85/514）。断奶前和断奶后的犊牛之间在流行率上未观察到显著差异。对ITS核苷酸序列进行的序列分析鉴定出6种已知基因型（BEB4、CC4、D、I、J和EbpC），其中5种先前在人类中检测到。基因型J是最常见的基因型（57/85），在11个养殖场中被鉴定出来。奶牛犊中具有人畜共患性的肠道微孢子虫基因型的高流行率表明，它们是人类人畜共患感染的潜在来源。
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