Benedik M J
Department of Biology, Texas A&M University, College Station 77843-3258.
Mol Gen Genet. 1989 Aug;218(2):353-4. doi: 10.1007/BF00331289.
This report demonstrates high efficiency transduction of enteric bacteria using single strand plasmids packaged in M13 phage capsids. Transformation by plasmid DNA is usually a very inefficient process in many enteric bacteria other than Escherichia coli K12. Plasmids carrying an M13 origin of replication can be replicated and packaged when cells carrying such plasmids are infected with M13 or a derivative helper phage. By introducing an F' plasmid into E. coli, Serratia marcescens, Citrobacter freundii, and Enterobacter aerogenes, these species can now be infected at high efficiency with M13 phage and with packaged single strand plasmids, yielding an efficient method to introduce cloned DNA fragments into these bacteria. The titer of colony forming units in a lysate was essentially equivalent in all the bacteria, demonstrating an equal efficiency of transduction of these other enteric bacteria compared to E. coli.
本报告展示了使用包装在M13噬菌体衣壳中的单链质粒对肠道细菌进行高效转导。在除大肠杆菌K12之外的许多肠道细菌中，质粒DNA转化通常是一个效率非常低的过程。携带M13复制起点的质粒在携带此类质粒的细胞被M13或衍生辅助噬菌体感染时能够进行复制和包装。通过将F'质粒引入大肠杆菌、粘质沙雷氏菌、弗氏柠檬酸杆菌和气生肠杆菌，现在这些菌种能够被M13噬菌体和包装好的单链质粒高效感染，从而产生了一种将克隆的DNA片段导入这些细菌的有效方法。裂解物中菌落形成单位的滴度在所有细菌中基本相同，表明与大肠杆菌相比，这些其他肠道细菌的转导效率相同。
