Liu Xin, Zhang Cuicui, Li Xiaogang, Tu Jumin
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
Shanxi Rice Research Institute , Dongta Road 356, Hanzhong, 723000 , China.
Breed Sci. 2016 Sep;66(4):591-598. doi: 10.1270/jsbbs.16014. Epub 2016 Jul 27.
Gene pyramiding is an efficient approach for the genetic improvement of multiple agronomic traits simultaneously. In this study, we pyramided two foreign genes, driven by the rice promoter, and (), driven by the endosperm-specific () promoter, into the elite cultivar 9311. These two genes were chosen in an attempt to enhance insect-resistance and Lysine (Lys) content. In the pyramided line, the foreign gene was efficiently expressed in the leaves and stems, and exhibited highly efficient resistance to striped stem borer (SSB, Walker) in the laboratory and rice leaf folder (RLF, Guenee) in the field. Furthermore, the gene was highly expressed in the endosperm and produced a remarkable increase of Lys content in the seeds of the pyramided line. The data from field trials demonstrated that most of the agronomic traits including yield were well maintained in the pyramided line compared to the parental control. These results strongly suggest that the foreign and genes have remarkable application potential in rice, and the resultant pyramided line serves as an ideal bridge material for the improvement of insect-resistance and high Lys rice in the future.
基因聚合是一种同时对多个农艺性状进行遗传改良的有效方法。在本研究中，我们将由水稻启动子驱动的两个外源基因以及由胚乳特异性启动子驱动的（）导入优良品种9311中。选择这两个基因是为了增强抗虫性和赖氨酸（Lys）含量。在聚合系中，外源基因在叶片和茎中高效表达，在实验室对二化螟（SSB，Walker）和田间对稻纵卷叶螟（RLF，Guenee）表现出高效抗性。此外，基因在胚乳中高表达，使聚合系种子中的赖氨酸含量显著增加。田间试验数据表明，与亲本对照相比，聚合系的大多数农艺性状包括产量都得到了良好保持。这些结果有力地表明，外源和基因在水稻中具有显著的应用潜力，所得聚合系可作为未来改良抗虫性和高赖氨酸水稻的理想桥梁材料。
