Liu Xin, Zhang Jiwen, Zhang Cuicui, Wang Liangchao, Chen Hao, Zhu Zengrong, Tu Jumin
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
Breed Sci. 2015 Sep;65(4):333-9. doi: 10.1270/jsbbs.65.333. Epub 2015 Sep 1.
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding.
螟虫和卷叶螟是在全球水稻（Oryza sativa L.）生产中造成严重危害的主要害虫。我们通过与华恢1号（带有cry1Ab/1Ac基因的优良品系）进行有性杂交，培育出了首个带有cry1Ab/1Ac苏云金芽孢杆菌（Bt）基因的光温敏雄性不育（PTSMS）水稻208S。新型208S及其杂交种对螟虫和卷叶螟表现出高而稳定的抗性，叶绿素组织中Cry1Ab/1Ac蛋白的含量达到了与供体相同的水平，且在非叶绿素组织中积累较少。在208S及其衍生杂交种中未观察到Bt基因的显性剂量效应。育性转换性状分析表明，208S在长日照/高温条件下完全不育，但在短日照/低温条件下部分可育。208S具有优良的稻米品质和良好的配合力，未观察到Bt（cry1Ab/1Ac）对其育性和农艺性状有负面影响。此外，与华恢1号不同，208S作为雄性不育系在Bt转基因过程中未出现育性下降的情况。因此，208S在两系杂交抗虫生产中具有很大的应用价值，也可作为水稻Bt转基因育种的桥梁材料。
