Peng Yanchun, Yu Zitong, Islam Shahidul, Zhang Yujuan, Wang Xiaolong, Lei Zhensheng, Yu Kan, Sun Dongfa, Ma Wujun
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
Breed Sci. 2016 Sep;66(4):646-652. doi: 10.1270/jsbbs.16050. Epub 2016 Aug 11.
Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results indicated that 17 alleles at three loci: , and were identified, resulting in 87 different allele combinations. Of the 17 alleles detected at all the loci, five belonged to , seven to and five to locus. MALDI-TOF-MS indicated was present in 72.8%, in 8.4%, in 8.4%, in 5.2% and in 3.6% lines. Seven types of alleles were identified at the locus: (25.5%), (21.3%), (16.9%), (13.8%), (8.4%), (8.2%), and (5.2%). Five types of alleles were detected: (58.4%), (22.6%), (15.5%), (3.3%) and (0.2%). Four new alleles that showed abnormal MALDI-TOF spectrum patterns were identified at the and loci. A detailed study is needed to further characterize these alleles and their potential usage for wheat improvement.
低分子量谷蛋白亚基是小麦贮藏蛋白的重要组成部分，在决定普通小麦的最终使用品质方面起着重要作用。采用一种新建立的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）方法，对从中国长江流域收集的478份面包小麦地方品种进行了分析。结果表明，在三个位点（Glu-A3、Glu-B3和Glu-D3）鉴定出17个等位基因，产生了87种不同的等位基因组合。在所有位点检测到的17个等位基因中，5个属于Glu-A3位点，7个属于Glu-B3位点，5个属于Glu-D3位点。MALDI-TOF-MS分析表明，72.8%的品系中存在Glu-A3位点的等位基因，8.4%的品系中存在Glu-B3位点的等位基因，8.4%的品系中存在Glu-D3位点的等位基因，5.2%的品系中存在另一种等位基因，3.6%的品系中存在又一种等位基因。在Glu-B3位点鉴定出7种等位基因类型：Glu-B3c（25.5%）、Glu-B3i（21.3%）、Glu-B3j（16.9%）、Glu-B3h（13.8%）、Glu-B3g（8.4%）、Glu-B3f（8.2%）和Glu-B3e（5.2%）。检测到5种Glu-D3等位基因类型：Glu-D3a（58.
