Hu Xin, Peng Yanchun, Ren Xifeng, Peng Junhua, Nevo Eviatar, Ma Wujun, Sun Dongfa
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Australia-China Joint Centre for Wheat Improvement, State Agriculture Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, WA 6150, Australia.
Breed Sci. 2018 Dec;68(5):524-535. doi: 10.1270/jsbbs.18085. Epub 2018 Nov 17.
Low molecular weight glutenin subunits (LMW-GS) play an important role in determining the bread-making characteristics of dough in the end-use quality of wheat. In this study, A total of 149 worldwide-originated durum wheat were used to analyze the composition of LMW-GS using MALDI-TOF-MS. Based on the allelic variation of glutenin subunits, the genetic diversity was evaluated for the 149 durum wheat. Five types of alleles were identified at the locus with , , , and accounting for 43.0%, 16.1%, 12.8%, 10.1% and 7.4 % of the accessions, respectively. Five types of alleles were identified at the locus: (60.4%), (6.0%), (6.0%), (2.7%) and (0.7%). Two novel alleles encoding abnormal subunits 40500 Da and 41260 Da were identified at the and loci, respectively. Further studies are needed to match these novel alleles to previously discovered novel alleles. Moreover, the genetic diversity analysis indicated that great genetic variation existed in durum wheat among encoding loci of glutenin subunits, released periods of varieties and different geographical origins. The results provide more important information of potential germplasm for the improvement of durum wheat and common wheat.
低分子量谷蛋白亚基（LMW-GS）在决定小麦最终用途品质中面团的制面包特性方面发挥着重要作用。在本研究中，共使用了149份来自世界各地的硬粒小麦，采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析LMW-GS的组成。基于谷蛋白亚基的等位变异，对这149份硬粒小麦的遗传多样性进行了评估。在该位点鉴定出了5种等位基因，分别为、、、和，占种质的比例分别为43.0%、16.1%、12.8%、10.1%和7.4%。在该位点鉴定出了5种等位基因：（60.4%）、（6.0%）、（6.0%）、（2.7%）和（0.7%）。分别在和位点鉴定出了两个编码异常亚基40500 Da和41260 Da的新等位基因。需要进一步研究将这些新等位基因与先前发现的新等位基因进行匹配。此外，遗传多样性分析表明，硬粒小麦在谷蛋白亚基编码位点、品种发布时期和不同地理起源之间存在很大的遗传变异。这些结果为硬粒小麦和普通小麦的改良提供了更重要的潜在种质信息。
