阿尔及利亚采集的血清中抗戊型肝炎病毒抗体对1型戊型肝炎病毒衍生抗原的结合偏好性

Binding Preference of Anti-HEV Antibodies in Sera Collected in Algeria for Antigens Derived From HEV Genotype 1‏.

作者信息

Behloul Nouredine, Zhang Min, Meng Jihong

机构信息

Department of Microbiology and Immunology, Southeast University School of Medicine, Nanjing, China.

Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

出版信息

Hepat Mon. 2016 Jul 20;16(8):e35312. doi: 10.5812/hepatmon.35312. eCollection 2016 Aug.

DOI:10.5812/hepatmon.35312

PMID:27795723

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5070561/

Abstract

BACKGROUND

Two hepatitis E virus (HEV) outbreaks occurred in Algeria (1979 - 1980 and 1987 - 1988). However, to date, no study on the prevalence of anti-HEV antibodies has been conducted in Algeria, and the genotype of the circulating strains remains unclear.

OBJECTIVES

This study was conducted to investigate the presence of anti- HEV antibodies among outpatients and blood donors in three different hospitals in Northern Algeria and to determine the genotype of the circulating strains through the characterization of the immunoreactivity of anti-HEV antibodies.

METHODS

A total of 590 blood samples (379 from blood donors and 211 from outpatients) were collected in three health facilities in Northern Algeria and assessed for anti-HEV antibodies using an in-house double-antigen sandwich immunoassay. HEV open reading frame 2 recombinant proteins p166 (aa 452 - 617) generated from the four HEV genotypes were used as antigens. The genotype of the strains circulating in Algeria was predicted by an indirect ELISA by assessing the anti-HEV antibodies in serially diluted positive sera using the different p166 proteins.

RESULTS

Anti-HEV antibodies were detected in 20.17% of the samples. A significant correlation was found between the age of the subjects and the presence of anti-HEV antibodies (P < 0.001). Among blood donors, 83 (21.9%) were diagnosed positive for anti-HEV antibodies with two cases weakly positive for anti-HEV IgM antibodies. Moreover, 9.9% of the subjects aged less than 25 years old (born after the last HEV outbreak) were positive for anti-HEV antibodies. The indirect ELISA revealed that the anti-HEV antibodies within the positive sera reacted more strongly against the p166 antigens generated from genotype 1.

CONCLUSIONS

The present findings reveal a relatively high presence of anti-HEV IgGs and clearly indicate that HEV infection is still present in Northern Algeria. Further, the prediction of HEV genotype using different antigens generated from the different HEV genotypes shows that the causative strains are more likely to be of genotype 1.

摘要

背景

阿尔及利亚曾发生过两次戊型肝炎病毒（HEV）疫情（1979 - 1980年和1987 - 1988年）。然而，迄今为止，阿尔及利亚尚未开展关于抗HEV抗体流行率的研究，且流行毒株的基因型仍不清楚。

目的

本研究旨在调查阿尔及利亚北部三家不同医院的门诊患者和献血者中抗HEV抗体的存在情况，并通过抗HEV抗体免疫反应性的特征分析来确定流行毒株的基因型。

方法

在阿尔及利亚北部的三个卫生机构共采集了590份血样（379份来自献血者，211份来自门诊患者），并使用内部双抗原夹心免疫分析法评估抗HEV抗体。从四种HEV基因型产生的HEV开放阅读框2重组蛋白p166（氨基酸452 - 617）用作抗原。通过间接ELISA，使用不同的p166蛋白评估系列稀释阳性血清中的抗HEV抗体，预测阿尔及利亚流行毒株的基因型。

结果

在20.17%的样本中检测到抗HEV抗体。在受试者年龄与抗HEV抗体的存在之间发现了显著相关性（P < 0.001）。在献血者中，83人（21.9%）被诊断为抗HEV抗体阳性，其中2例抗HEV IgM抗体弱阳性。此外，年龄小于25岁（在最后一次HEV疫情之后出生）的受试者中有9.9%抗HEV抗体呈阳性。间接ELISA显示，阳性血清中的抗HEV抗体对基因型1产生的p166抗原反应更强。