Choi Ji-Woong, Kwon Tae-Yub, Hong Su-Hyung, Lee Heon-Jin
Department of Oral Microbiology and Immunology, School of Dentistry, Kyungpook National University, Daegu, Korea.
Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, Daegu, Korea.
Cell Biochem Biophys. 2018 Jun;76(1-2):293-301. doi: 10.1007/s12013-016-0770-5. Epub 2016 Oct 31.
MicroRNAs in eukaryotic cells are thought to control highly complex signal transduction and other biological processes by regulating coding transcripts, accounting for their important role in cellular events in eukaryotes. Recently, a novel class of bacterial RNAs similar in size [18-22 nucleotides (nt)] to microRNAs has been reported. Herein, we describe microRNAs, small RNAs from the oral pathogen Streptococcus sanguinis. The bacteria are normally present in the oral cavities and cause endocarditis by contaminating bloodstreams. Small RNAs were analyzed by deep sequencing. Selected highly expressed small RNAs were further validated by real-time polymerase chain reaction and northern blot analyses. We found that skim milk supplement changed the expression of small RNAs S.S-1964 in tandem with the nearby SSA_0513 gene involved in vitamin B conversion. We furthermore observed small RNAs secreted via bacterial membrane vesicles. Although their precise function remains unclear, secretable small RNAs may represent an entirely new area of study in bacterial genetics.
真核细胞中的微小RNA被认为通过调控编码转录本控制高度复杂的信号转导及其他生物学过程，这也解释了它们在真核生物细胞事件中的重要作用。最近，已报道了一类新型细菌RNA，其大小[18 - 22个核苷酸（nt）]与微小RNA相似。在此，我们描述了来自口腔病原体血链球菌的微小RNA及小RNA。这种细菌通常存在于口腔中，可通过污染血流导致心内膜炎。通过深度测序分析小RNA。通过实时聚合酶链反应和Northern印迹分析进一步验证了选定的高表达小RNA。我们发现脱脂牛奶补充剂会与参与维生素B转化的附近SSA_0513基因协同改变小RNA S.S - 1964的表达。此外，我们观察到小RNA通过细菌膜泡分泌。尽管它们的确切功能尚不清楚，但可分泌的小RNA可能代表了细菌遗传学中一个全新的研究领域。
