Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu, Korea.
FEMS Microbiol Lett. 2012 Jan;326(2):131-6. doi: 10.1111/j.1574-6968.2011.02441.x. Epub 2011 Nov 8.
MicroRNAs (miRNAs) are important modulators of gene expression in eukaryotic cells. However RNAs of the same size in bacteria have not been specifically discussed previously. Here, we provide a library of miRNA-size RNAs (msRNAs), which were registered by deep sequencing in Streptococcus mutans. Bioinformatic analysis of the whole set revealed more than 900 individual msRNA species. The cellular content of selected msRNAs was verified by quantitative RT-PCR and Northern blotting. The high abundance and discrete size of the subset of registered msRNAs suggest their functional significance, although the precise biological role of the RNA species revealed in S. mutans, which is one of the principle causative agents of dental caries, has to be elucidated.
微小 RNA（miRNAs）是真核细胞中基因表达的重要调控因子。然而，以前尚未专门讨论细菌中大小相同的 RNA。在这里，我们提供了一个变形链球菌 miRNA 大小 RNA（msRNA）文库，该文库通过深度测序进行了注册。对整个文库的生物信息学分析揭示了超过 900 种不同的 msRNA 种类。通过定量 RT-PCR 和 Northern 印迹验证了选定的 msRNA 的细胞含量。所注册的 msRNA 亚组的高丰度和离散大小表明它们具有功能意义，尽管必须阐明在变形链球菌中发现的 RNA 种类的精确生物学作用，变形链球菌是导致龋齿的主要病原体之一。
