Shah Ankeeta, Qian Yingzhi, Weyn-Vanhentenryck Sebastien M, Zhang Chaolin
Department of Systems BiologyDepartment of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Bioinformatics. 2017 Feb 15;33(4):566-567. doi: 10.1093/bioinformatics/btw653.
UV cross-linking and immunoprecipitation (CLIP), followed by high-throughput sequencing, is a powerful biochemical assay that maps in vivo protein-RNA interactions on a genome-wide scale. The CLIP Tool Kit (CTK) aims at providing a set of tools for flexible, streamlined and comprehensive CLIP data analysis. This software package extends the scope of our original CIMS package.
The software is implemented in Perl. The source code and detailed documentation are available at http://zhanglab.c2b2.columbia.edu/index.php/CTK .
紫外线交联免疫沉淀法(CLIP),随后进行高通量测序,是一种强大的生化分析方法,可在全基因组范围内绘制体内蛋白质-RNA相互作用图谱。CLIP工具包(CTK)旨在提供一套工具,用于灵活、简化和全面的CLIP数据分析。该软件包扩展了我们原始CIMS软件包的范围。
该软件用Perl语言实现。源代码和详细文档可在http://zhanglab.c2b2.columbia.edu/index.php/CTK获取。