Campagna Maria Concetta, Di Schiavi Maria Teresa, Foti Marina, Mosconi Maria Cristina, Mattiolo Giuseppina, Cavallina Roberta
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana , Roma.
Microbiologia Alimentare, Laboratorio di Prevenzione ASL Milano.
Ital J Food Saf. 2014 Aug 28;3(3):1650. doi: 10.4081/ijfs.2014.1650.
Irradiation is a preservation technology used to improve the safety and hygienic quality of food. Aim of this study was to assess the applicability and validity of the microbiological screening method direct epifluorescence filter technique (DEFT)/aerobic plate count (APC) (EN 13783:2001) for the identification of irradiated herbs and spices. Tests on non-irradiated and irradiated samples of dried herbs and spices were performed. The method was based on the comparison of APC and count obtained using DEFT. In accordance with the standard reference, this method is not applicable to samples with APC<10 colony forming units (CFU)/g and this is its main limit. The results obtained in our laboratories showed that in 50% of cases of non-irradiated samples and in 96% of the samples treated with ionising radiation, the method was not applicable due to a value of CFU/g <10.
辐照是一种用于提高食品安全性和卫生质量的保鲜技术。本研究的目的是评估微生物筛选方法——直接荧光滤膜技术（DEFT）/需氧平板计数法（APC）（EN 13783:2001）用于鉴别辐照药草和香料的适用性和有效性。对干燥药草和香料的未辐照及辐照样品进行了测试。该方法基于APC与使用DEFT获得的计数的比较。根据标准参考文献，该方法不适用于APC<10菌落形成单位（CFU）/克的样品，这是其主要局限性。我们实验室获得的结果表明，在50%的未辐照样品和96%的电离辐射处理样品中，由于CFU/克值<10，该方法不适用。
