Baek Mi-Eun, Ameer Kashif, Jo Yunhee, Chung Namhyeok, Choi Minji, Kim Jung-Ok, Won Jaehee, Lee Sang-Han, Kwon Joong-Ho
1School of Food Science and Biotechnology and Food Bio-industry Research Institute, Kyungpook National University, Daegu, 41566 Korea.
Quality Certification Center, National Institute of Korean Medicine Development, Daegu, 41934 Korea.
Food Sci Biotechnol. 2019 Nov 1;29(5):705-715. doi: 10.1007/s10068-019-00701-w. eCollection 2020 May.
Medicinal herbs comprise of heavy microbial contaminations. This study aimed to assess microbial hazards including foodborne pathogens in 20 commercial medicinal herbs, Cnidii Rhizoma (C1-C10) and Alismatis Rhizoma (T1-T10) as well as to evaluate irradiation effects of E-beam on microbial load and detection chracteristics. Four samples (C5, C10, T1, T8) from both herbs with higher microbial load were selected for evaluating the irradiation effect of E-beam (up to 10 kGy) on microbial load and radiation-induced changes in detection markers by standard methods (Codex, Korean Food Code), such as direct epifluorescent filter technique/aerobic plate count (DEFT/APC), photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR). DEFT/APC revealed non-evidence of pre-sterilization of all samples. PSL differentiated irradiated samples (1, 5, and 10 kGy) of both herbs from non-irradiated (control: 0 kGy). Both TL and ESR methods validated PSL screening results by detecting radiation-induced markers from E-beam irradiated medicinal herbs.
草药存在严重的微生物污染问题。本研究旨在评估20种市售草药（蛇床子（C1 - C10）和泽泻（T1 - T10））中的微生物危害，包括食源性病原体，并评估电子束辐照对微生物负荷及检测特性的影响。从两种微生物负荷较高的草药中选取了四个样本（C5、C10、T1、T8），通过标准方法（食品法典委员会、韩国食品法典），如直接表面荧光过滤技术/需氧平板计数法（DEFT/APC）、光激发发光（PSL）、热释光（TL）和电子自旋共振（ESR），来评估电子束（高达10千戈瑞）对微生物负荷的辐照效果以及辐射诱导的检测标志物变化。DEFT/APC结果显示所有样本均无预灭菌迹象。PSL能够区分两种草药的辐照样本（1、5和10千戈瑞）与未辐照样本（对照：0千戈瑞）。TL和ESR方法通过检测电子束辐照草药中的辐射诱导标志物，验证了PSL筛选结果。
