Najioullah F, Freney J, Pellon G, Meugnier H, Dorleac E, Durand C, Fleurette J
Laboratoire de Bactériologie, Faculté de Médecine Alexis-Carrel, Lyon.
Pathol Biol (Paris). 1989 May;37(5):370-4.
Six antibiotics active on the cell-wall were tested against a methicillin resistant Staphylococcus aureus P18 strain at the start of exponential growth rate. The concentration was four-fold the MIC determined in a non-hypersaline liquid medium. The peptidoglycan amino-acid content of the various cell wall samples was determined. An antibiotic-free S. aureus P18 and the methicillin-sensitive Cowan I. S. aureus strain were used a controls. Beta lactam antibiotic treatment, oxacillin and cephalothin, induced an increase of alanine content which suggests that only secondary-transpeptidation did not occur. Similar results were obtained using vancomycin. Amino-acid content was not modified by bacitracin, which is in agreement with the results expected. The results obtained for fosfomycin and cefamandole were not easy to interpret.
在指数生长速率开始时，针对一株耐甲氧西林金黄色葡萄球菌P18菌株测试了六种对细胞壁有活性的抗生素。浓度为在非高盐液体培养基中测定的最低抑菌浓度（MIC）的四倍。测定了各种细胞壁样品的肽聚糖氨基酸含量。使用无抗生素的金黄色葡萄球菌P18和对甲氧西林敏感的考恩I型金黄色葡萄球菌菌株作为对照。β-内酰胺类抗生素治疗，即苯唑西林和头孢噻吩，导致丙氨酸含量增加，这表明仅未发生二次转肽作用。使用万古霉素也获得了类似结果。杆菌肽未改变氨基酸含量，这与预期结果一致。磷霉素和头孢孟多的结果不易解释。
