水合离子液体作为大肠杆菌中表达的聚集重组蛋白复性的液体伴侣。
Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli.
作者信息
Fujita K, Kajiyama M, Liu Y, Nakamura N, Ohno H
机构信息
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan and Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan.
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
出版信息
Chem Commun (Camb). 2016 Nov 10;52(92):13491-13494. doi: 10.1039/c6cc06999a.
We have succeeded in refolding of aggregated recombinant protein from Escherichia coli in hydrated ionic liquids. In cholinium dihydrogen phosphate containing a limited amount of water molecules, aggregated solid cellulase was dissolved and refolding was successfully carried out without further processing.
我们已成功在水合离子液体中对来自大肠杆菌的聚集重组蛋白进行复性。在含有有限数量水分子的磷酸二氢胆碱中，聚集的固体纤维素酶被溶解，并且无需进一步处理就成功进行了复性。
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