Kaul Deepak, Hussain Aashiq
Indian J Biochem Biophys. 2009 Jun;46(3):237-40.
HIV-1 encoded microRNA hiv1-miR-H1 is known to induce CD4+ lymphopenia through its ability to downregulate cellular AATF gene. The present study directed to examine the target sites of this miRNA on AATF gene revealed the existence of a novel miRNA designated as hmiR-che-1 which had the inherent capacity to target HIV-1 genome especially regions coding for hiv1-miR-H1 as well as Vpr gene. Further, the expression of AATF gene coupled with its encoded microRNA hmiR-che-1 exhibited characteristic antagonism with the expression of hiv1-miR-H1 within the lymphocytes, derived from asymptomatic as well as symptomatic AIDS subjects. Based upon these observations, we propose that the widely recognised HIV-1 latency in CD4+ T-lymphocytes may arise, because of the orchestrated balance that may exist between the expression levels of hiv1-miR-H1 and hmiR-che-1 within lymphocytes infected with HIV-1.
已知HIV-1编码的微小RNA hiv1-miR-H1能够通过下调细胞AATF基因来诱导CD4+淋巴细胞减少。本研究旨在检测该微小RNA在AATF基因上的靶位点，结果发现一种新的微小RNA，命名为hmiR-che-1，它具有靶向HIV-1基因组的内在能力，特别是编码hiv1-miR-H1以及Vpr基因的区域。此外，AATF基因及其编码的微小RNA hmiR-che-1的表达，与来自无症状以及有症状艾滋病患者的淋巴细胞内hiv1-miR-H1的表达呈现出特征性的拮抗作用。基于这些观察结果，我们提出，在感染HIV-1的淋巴细胞内，hiv1-miR-H1和hmiR-che-1表达水平之间可能存在的协调平衡，或许是导致CD4+ T淋巴细胞中广泛认可的HIV-1潜伏状态产生的原因。
