State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Front Immunol. 2023 Jun 2;14:1204730. doi: 10.3389/fimmu.2023.1204730. eCollection 2023.
Viruses are simple yet highly pathogenic microorganisms that parasitize within cells and pose serious threats to the health, economic development, and social stability of both humans and animals. Therefore, it is crucial to understand the dynamic mechanism of virus infection in hosts. One effective way to achieve this is through virus tracking technology, which utilizes fluorescence imaging to track the life processes of virus particles in living cells in real-time, providing a comprehensively and detailed spatiotemporal dynamic process and mechanism of virus infection. This paper provides a broad overview of virus tracking technology, including the selection of fluorescent labels and virus labeling components, the development of imaging microscopes, and its applications in various virus studies. Additionally, we discuss the possibilities and challenges of its future development, offering theoretical guidance and technical support for effective prevention and control of the viral disease outbreaks and epidemics.
病毒是简单而又极具致病性的微生物，它们寄生在细胞内，对人类和动物的健康、经济发展和社会稳定构成严重威胁。因此，了解病毒在宿主中的动态感染机制至关重要。一种有效的方法是利用病毒跟踪技术，该技术利用荧光成像实时跟踪病毒颗粒在活细胞中的生命过程，提供病毒感染的全面、详细的时空动态过程和机制。本文广泛概述了病毒跟踪技术，包括荧光标记物和病毒标记成分的选择、成像显微镜的发展及其在各种病毒研究中的应用。此外，我们还讨论了其未来发展的可能性和挑战，为有效预防和控制病毒病暴发和流行提供了理论指导和技术支持。
