Ding Dong, Zhang Yaodong, Yang Renjie, Wang Xing, Ji Guwei, Huo Liqun, Shao Zicheng, Li Xiangcheng
Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, China.
Biomed Res Int. 2016;2016:7618342. doi: 10.1155/2016/7618342. Epub 2016 Oct 11.
. To investigate the expression of miR-940 in the hepatocellular carcinoma (HCC) and its impact on function and biological mechanism in the HCC cells. . Quantitative RT-PCR analysis was used to quantify miR-940 expression in 46 cases of tissues and cells. Transfection of HCC cell lines was performed by miR-940 mimics; the abilities of invasion and migration were assessed through Transwell array. Western blot represents the alteration in expression of CXCR2 by miR-940 mimics. . miR-940 expression was decreased significantly in the HCC tissues and the relevant cell lines. miR-940 upregulation suppressed the invasion and migration of HCC cells in vitro. Furthermore, the CXCR2 was downregulated to suppress invasion and migration after miR-940 mimics. Moreover, decreased miR-940 expression was negatively correlated with Edmondson grade ( = 0.008), tumor microsatellite or multiple tumors ( = 0.04), vascular invasion ( = 0.035), and recurrence and metastasis ( = 0.038). Kaplan-Meier analysis demonstrated that decreased miR-940 expression contributed to poor overall survival ( < 0.05). . Our findings present that miR-940 acts as a pivotal adaptor of CXCR2 and its transcription downregulated CXCR2 expression to decrease HCC invasion and migration in vitro. Our study suggests that miR-940 may be a novel poor prognostic biomarker for HCC.
研究miR-940在肝细胞癌（HCC）中的表达及其对HCC细胞功能和生物学机制的影响。采用定量逆转录聚合酶链反应（RT-PCR）分析46例组织和细胞中miR-940的表达。通过miR-940模拟物转染HCC细胞系；通过Transwell小室检测侵袭和迁移能力。蛋白质免疫印迹法检测miR-940模拟物对CXCR2表达的影响。HCC组织及相关细胞系中miR-940表达显著降低。上调miR-940可抑制HCC细胞的体外侵袭和迁移。此外，miR-940模拟物转染后CXCR2表达下调，从而抑制侵袭和迁移。而且，miR-940表达降低与Edmondson分级（P = 0.008）、肿瘤微卫星或多发肿瘤（P = 0.04）、血管侵犯（P = 0.035）以及复发和转移（P = 0.038）呈负相关。Kaplan-Meier分析表明，miR-940表达降低导致总体生存率较差（P < 0.05）。我们的研究结果表明，miR-940是CXCR2的关键调节因子，其转录下调CXCR2表达，从而降低HCC细胞的体外侵袭和迁移。我们的研究提示，miR-940可能是HCC一种新的预后不良生物标志物。
