Irkutsk State University, Institute of Biology, Irkutsk, 664003, Russia.
Karelian Research Centre of Russian Academy of Sciences, Institute of Biology, Petrozavodsk, 185035, Russia.
Sci Rep. 2016 Nov 3;6:36427. doi: 10.1038/srep36427.
Remote in vivo scanning of physiological parameters is a major trend in the development of new tools for the fields of medicine and animal physiology. For this purpose, a variety of implantable optical micro- and nanosensors have been designed for potential medical applications. At the same time, the important area of environmental sciences has been neglected in the development of techniques for remote physiological measurements. In the field of environmental monitoring and related research, there is a constant demand for new effective and quick techniques for the stress assessment of aquatic animals, and the development of proper methods for remote physiological measurements in vivo may significantly increase the precision and throughput of analyses in this field. In the present study, we apply pH-sensitive microencapsulated biomarkers to remotely monitor the pH of haemolymph in vivo in endemic amphipods from Lake Baikal, and we compare the suitability of this technique for stress assessment with that of common biochemical methods. For the first time, we demonstrate the possibility of remotely detecting a change in a physiological parameter in an aquatic organism under ecologically relevant stressful conditions and show the applicability of techniques using microencapsulated biomarkers for remote physiological measurements in environmental monitoring.
远程活体扫描生理参数是医学和动物生理学领域新工具发展的主要趋势。为此，已经设计了各种可植入的光学微纳传感器，以用于潜在的医学应用。与此同时，在远程生理测量技术的发展中，重要的环境科学领域被忽视了。在环境监测及相关研究领域，人们一直需要新的、有效的、快速的水生动物应激评估技术，而开发合适的活体远程生理测量方法可能会显著提高该领域分析的精度和通量。在本研究中，我们应用 pH 敏感的微囊化生物标志物来远程监测贝加尔湖特有片脚类动物血淋巴的 pH 值，并将该技术用于应激评估的适用性与常见生化方法进行比较。我们首次证明了在生态相关的胁迫条件下远程检测水生生物生理参数变化的可能性，并展示了使用微囊化生物标志物进行远程生理测量在环境监测中的适用性。
