Popov Alexey, Timofeyev Maxim, Bykov Alexander, Meglinski Igor
VTT Technical Research Centre of Finland, 90590 Oulu, Finland.
Institute of Biology, Irkutsk State University, Irkutsk 664003, Russia.
iScience. 2022 Jun 9;25(7):104568. doi: 10.1016/j.isci.2022.104568. eCollection 2022 Jul 15.
Growing anthropogenic activities are significantly influencing the environment and especially aquatic ecosystems. Therefore, there is an increasing demand to develop techniques for monitoring and assessing freshwater habitat changes so that interventions can prevent irrevocable damage. We explore an approach for screening the temperature-induced stress experienced by aquatic organisms owing to environmental variations. Luminescent spectra of upconversion [Y2O3: Yb, Er] particles embedded within shrimps are measured, while ambient temperature gradient is inducing stress conditions. The inverse linear dependence of the logarithmic ratio of the luminescence intensity provides an effective means for temperature evaluation inside aquatic species . The measured luminescence shows high photostability on the background of the complete absence of biotissues' autofluorescence, as well as no obscuration of the luminescence signal from upconversion particles. Current approach of hybrid sensing has a great potential for monitoring variations in aquatic ecosystems driven by climate changes and pollution.
日益增长的人类活动正在对环境，尤其是水生生态系统产生重大影响。因此，开发监测和评估淡水栖息地变化的技术的需求日益增加，以便采取干预措施防止不可挽回的损害。我们探索了一种方法，用于筛选由于环境变化而导致水生生物所经历的温度诱导应激。在环境温度梯度诱导应激条件的同时，测量嵌入虾体内的上转换[Y2O3:Yb,Er]颗粒的发光光谱。发光强度对数比的反线性依赖性为评估水生物种内部的温度提供了一种有效手段。所测量的发光在完全没有生物组织自发荧光的背景下显示出高光稳定性，并且来自上转换颗粒的发光信号也没有被遮挡。当前的混合传感方法在监测由气候变化和污染驱动的水生生态系统变化方面具有巨大潜力。
