柠檬酸盐稳定的镧系元素掺杂纳米颗粒：脑渗透及与免疫细胞和神经元的相互作用
Citrate-stabilized lanthanide-doped nanoparticles: brain penetration and interaction with immune cells and neurons.
作者信息
Portioli Corinne, Pedroni Marco, Benati Donatella, Donini Marta, Bonafede Roberta, Mariotti Raffaella, Perbellini Luigi, Cerpelloni Marzia, Dusi Stefano, Speghini Adolfo, Bentivoglio Marina
机构信息
Department of Neuroscience, Biomedicine & Movement Sciences, University of Verona, Verona, Italy.
Department of Biotechnology, University of Verona, Verona, Italy.
出版信息
Nanomedicine (Lond). 2016 Dec;11(23):3039-3051. doi: 10.2217/nnm-2016-0297. Epub 2016 Nov 4.
AIM
To unravel key aspects of the use of lanthanide-doped nanoparticles (NPs) in biomedicine, the interaction with immune and brain cells.
MATERIALS & METHODS: Effects of citrate-stabilized CaF and SrF: Yb, Er NPs (13-15 nm) on human dendritic cells and neurons were assessed in vitro. In vivo distribution was analyzed in mice at tissue and ultrastructural levels, and with glia immunophenotyping.
RESULTS
The NPs did not elicit dendritic cell activation and were internalized by cultured neurons, without viability changes. After intravenous injection, NPs were found in the brain parenchyma, without features of glial neuroinflammatory response.
CONCLUSION
Lanthanide-doped NPs do not activate cells protagonists of systemic and brain immune responses, are endocytosed by neurons and can cross an intact blood-brain barrier.
目的
揭示镧系元素掺杂纳米颗粒（NPs）在生物医学中的关键应用方面，以及与免疫细胞和脑细胞的相互作用。
材料与方法
在体外评估柠檬酸盐稳定的CaF和SrF:Yb、Er NPs（13 - 15纳米）对人树突状细胞和神经元的影响。在小鼠体内，从组织和超微结构水平以及胶质细胞免疫表型分析其体内分布。
结果
这些纳米颗粒未引发树突状细胞激活，被培养的神经元内化，且细胞活力无变化。静脉注射后，在脑实质中发现纳米颗粒，无胶质细胞神经炎症反应特征。
结论
镧系元素掺杂纳米颗粒不会激活全身和脑免疫反应的细胞主角，可被神经元内吞，并能穿过完整的血脑屏障。
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