Knight L C, Maurer A H, Ammar I A, Shealy D J, Mattis J A
Temple University School of Medicine and Hospital, Philadelphia, PA.
J Nucl Med. 1988 Apr;29(4):494-502.
Monoclonal antibody 59D8 developed by Hui et al., binds to fibrin but not fibrinogen. An 111In-labeled Fab fragment of 59D8 was studied in vitro and in animal models to evaluate its potential for imaging thrombi and emboli in man. Rabbits and dogs were used as models for studying thrombus uptake in vivo. Thrombi and emboli up to 4 days old were successfully visualized at 4-24 hr postinjection in five of eight rabbits. In dogs, 0.5-hr-old and 24-hr-old thrombi were successfully imaged at 24 hr in six of eight animals, and 3/6 of these were positive at 3-4 hr postinjection. Thrombus-to-blood ratios in the dogs averaged 7.1 +/- 1.3. The findings suggest this antibody may be useful for imaging thrombi in man.
Hui等人研发的单克隆抗体59D8可与纤维蛋白结合，但不与纤维蛋白原结合。对59D8的111铟标记Fab片段进行了体外和动物模型研究，以评估其对人体血栓和栓子成像的潜力。使用兔子和狗作为体内研究血栓摄取的模型。在八只兔子中的五只中，注射后4 - 24小时成功观察到了长达4天的血栓和栓子。在狗身上，八只动物中的六只在24小时成功对0.5小时和24小时的血栓进行了成像，其中六只中的三只在注射后3 - 4小时呈阳性。狗的血栓与血液比率平均为7.1±1.3。这些发现表明该抗体可能对人体血栓成像有用。
