Meneguetti Dionatas Ulises de Oliveira, Lima Renato Abreu, Hurtado Fernanda Bay, Passarini Guilherme Matos, Macedo Sharon Rose Aragão, Barros Neuza Biguinati de, Oliveira Flávio Augusto de Souza, Medeiros Patrícia Soares de Maria de, Militão Júlio Sancho Linhares Teixeira, Nicolete Roberto, Facundo Valdir Alves
Programa de Pós Graduação em Biologia Experimental, Universidade Federal de Rondônia, Porto Velho, Rondônia, Brazil.
Laboratório de Fisiofarmacologia, Colégio de Aplicação, Universidade Federal do Acre, Rio Branco, Acre, Brazil.
Rev Soc Bras Med Trop. 2016 Sep-Oct;49(5):579-585. doi: 10.1590/0037-8682-0156-2016.
Maytenus guianensis is a member of the Celastraceae family that is used in traditional medicine, particularly for its anti-parasitic and anti-cancer effects. To explore the ethnopharmacological potential of this plant, the present study was designed to screen the in vitro antileishmanial activities of extracts and compounds isolated from M. guianensis.
Maytenus guianensis stems and leaves were extracted in acetone, followed by the preparation of eluates and isolation of secondary metabolites using chromatography on a glass column with silica gel as the fixed phase. The chemical components were identified using spectroscopic methods, including one- and two-dimensional nuclear magnetic resonance of hydrogen-1 and carbon-13, mass spectroscopy, and infrared spectroscopy. The anti-Leishmania amazonensis activities of these eluates and compounds were evaluated by direct promastigote counting and viability assays.
It was found that the hexane bark eluate produced the strongest anti-L. amazonensis effect, with 90-100% inhibition of the promastigote form. The isolated metabolite that produced the best result was tingenone B, followed by a compound formed by the union of tingenone and tingenone B (80-90% inhibition).
Maytenus guianensis shows anti-parasite activity that warrants further investigation to determine the mechanisms underlying this antileishmanial effect and to evaluate the pharmacological potential of these eluates and isolated secondary metabolites, while minimizing any adverse effects.
圭亚那美登木是卫矛科的一员，在传统医学中有所应用，尤其因其具有抗寄生虫和抗癌作用。为了探索这种植物的民族药理学潜力，本研究旨在筛选从圭亚那美登木中分离得到的提取物和化合物的体外抗利什曼原虫活性。
用丙酮提取圭亚那美登木的茎和叶，然后制备洗脱液，并使用以硅胶为固定相的玻璃柱色谱法分离次生代谢产物。利用光谱方法鉴定化学成分，包括一维和二维氢-1和碳-13核磁共振、质谱和红外光谱。通过直接前鞭毛体计数和活力测定评估这些洗脱液和化合物对亚马逊利什曼原虫的活性。
发现己烷树皮洗脱液产生了最强的抗亚马逊利什曼原虫效果，对前鞭毛体形式的抑制率为90 - 100%。产生最佳结果的分离代谢产物是丁香酮B，其次是由丁香酮和丁香酮B结合形成的一种化合物（抑制率为80 - 90%）。
圭亚那美登木显示出抗寄生虫活性，值得进一步研究以确定这种抗利什曼原虫作用的潜在机制，并评估这些洗脱液和分离的次生代谢产物的药理学潜力，同时尽量减少任何不良反应。
