NEAT1通过微小RNA-335-5p/c-甲硫氨酸轴调节胰腺癌细胞的生长、侵袭和迁移。
NEAT1 regulates pancreatic cancer cell growth, invasion and migration though mircroRNA-335-5p/c-met axis.
作者信息
Cao Jia, Zhang Yi, Yang Jiachun, He Sijia, Li Mingming, Yan Shiyan, Chen Ying, Qu Chunying, Xu Leiming
机构信息
Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Shanghai 200092, China.
出版信息
Am J Cancer Res. 2016 Oct 1;6(10):2361-2374. eCollection 2016.
Abstract
NEAT1 has been reported to affect cancer progression, which was subsequently confirmed in multiple cancers. Hsa-miRNA-335-5p (miR-335-5p) has recently been identified as an anticancer agent in various organs. However, the relationship between NEAT1 and miR-335-5p remains poorly understood. In this study, we investigated the effects of NEAT1 and miR-335-5p on development of pancreatic cancer. The ectopic expression of miR-335-5p in pancreatic cancer cell lines significantly suppressed cell growth by inhibiting c-met. In addition, downregulating NEAT1 upregulates miR-335-5p. Taken together, our results demonstrate that the NEAT1/miR-335-5p/c-met axis plays a pivotal role in pancreatic cancer by regulating the proliferation, metastasis, and apoptosis of pancreatic cancer cells in vivo and in vitro.
摘要
据报道，NEAT1会影响癌症进展，随后在多种癌症中得到证实。Hsa-miRNA-335-5p（miR-335-5p）最近被确定为各器官中的一种抗癌剂。然而，NEAT1与miR-335-5p之间的关系仍知之甚少。在本研究中，我们调查了NEAT1和miR-335-5p对胰腺癌发展的影响。miR-335-5p在胰腺癌细胞系中的异位表达通过抑制c-met显著抑制了细胞生长。此外，下调NEAT1会上调miR-335-5p。综上所述，我们的结果表明，NEAT1/miR-335-5p/c-met轴通过在体内和体外调节胰腺癌细胞的增殖、转移和凋亡，在胰腺癌中起关键作用。
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