Lee In-Chul, Bae Jong-Sup
Department of Cosmetic Science and Technology, Seowon University, Cheongju, 28674, Republic of Korea.
College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University, 80 Dahak-ro, Daegu, 41566, Republic of Korea.
Arch Pharm Res. 2017 Feb;40(2):258-267. doi: 10.1007/s12272-016-0856-z. Epub 2016 Nov 8.
Previous reports suggest that human endothelial cells-derived PolyPhosphate (PolyP) is one of the pro-inflammatory mediators. As a well-known red pigment and found in plants, Pelargonidin (PEL) has been known to have several biological activates which are beneficial for human health. This study was undertaken to investigate whether PEL can modulate PolyP-mediated inflammatory responses in human umbilical vein endothelial cells (HUVECs) and in mice. The anti-inflammatory activities of PEL were determined by measuring permeability, leukocytes adhesion and migration, and activation of pro-inflammatory proteins in PolyP-activated HUVECs and mice. In addition, the beneficial effects of PEL on survival rate in PolyP-injected mice. We found that PEL inhibits PolyP-mediated barrier disruption, the expressions of cell adhesion molecules, and leukocyte to HUVEC adhesion/migration. Interestingly, PolyP-induced NF-κB activation and the productions of TNF-α and IL-6 were inhibited by PEL in HUVECs. These anti-inflammatory functions of PEL were confirmed in PolyP injected mice. These results suggest that PEL have therapeutic potential for various systemic inflammatory diseases.
先前的报道表明，人内皮细胞衍生的多聚磷酸盐（PolyP）是促炎介质之一。作为一种广为人知的红色色素且存在于植物中，天竺葵素（PEL）已知具有多种对人体健康有益的生物活性。本研究旨在调查PEL是否能调节人脐静脉内皮细胞（HUVECs）和小鼠中PolyP介导的炎症反应。通过测量PolyP激活的HUVECs和小鼠中的通透性、白细胞黏附和迁移以及促炎蛋白的激活来确定PEL的抗炎活性。此外，还研究了PEL对PolyP注射小鼠存活率的有益影响。我们发现PEL可抑制PolyP介导的屏障破坏、细胞黏附分子的表达以及白细胞与HUVEC的黏附/迁移。有趣的是，在HUVECs中，PEL可抑制PolyP诱导的NF-κB激活以及TNF-α和IL-6的产生。PEL的这些抗炎功能在注射PolyP的小鼠中得到了证实。这些结果表明，PEL对各种全身性炎症疾病具有治疗潜力。
