Reymão Tammy Kathlyn Amaral, Hernandez Juliana das Merces, Costa Samya Thalita Picanço da, Sousa Maísa Silva de, Oliveira Darleise de Souza, Silva Luciana Damascena da, Bandeira Renato da Silva, Lima Ian Carlos Gomes de, Soares Luana da Silva, Mascarenhas Joana Darc Pereira, Gabbay Yvone Benchimol
Evandro Chagas Institute, Postgraduate Program in Virology. Ananindeua, Pará, Brazil. E-mails:
University of the State of Pará, Postgraduate Program in Parasitic, Biology of the Amazon. Belém, Pará, Brazil. E-mail:
Rev Inst Med Trop Sao Paulo. 2016 Nov 3;58:81. doi: 10.1590/S1678-9946201658081.
Sapoviruses (SaVs) are responsible for acute gastroenteritis in humans, especially children and the elderly. In Brazil, data on SaVs infections are very limited, especially in Northern Brazil. Here, we investigated the occurrence of SaVs in samples from hospitalized children under ten years old that presented acute gastroenteritis. Positive samples were genotyped and phylogenetic analysis was performed using prototype strains sequences obtained from GenBank database. In total, 156 fecal samples were screened by RT-PCR for SaVs. A positivity rate of 3.8% (6/156) was found in children under three years of age. Four genotypes were detected: GI.I, GI.2 and GII.2?-GII.4?/GII.4, suggesting a possible inter-genotypes recombination. Most infections (83.3%) occurred between August and September. The positivity was similar to that found in other countries and genotyping demonstrated the presence of distinct genotypes. To our knowledge, this is the first study reporting the circulation of SaVs in Manaus, state of Amazonas, Amazon region, Brazil.
札幌病毒（SaVs）可导致人类急性肠胃炎，尤其是儿童和老年人。在巴西，关于SaVs感染的数据非常有限，特别是在巴西北部地区。在此，我们调查了10岁以下患有急性肠胃炎的住院儿童样本中SaVs的出现情况。对阳性样本进行基因分型，并使用从GenBank数据库获得的原型菌株序列进行系统发育分析。总共通过RT-PCR对156份粪便样本进行了SaVs筛查。在3岁以下儿童中发现阳性率为3.8%（6/156）。检测到四种基因型：GI.I、GI.2和GII.2?-GII.4?/GII.4，提示可能存在基因型间重组。大多数感染（83.3%）发生在8月至9月之间。阳性率与其他国家的情况相似，基因分型表明存在不同的基因型。据我们所知，这是第一项报道巴西亚马逊地区亚马孙州玛瑙斯市SaVs传播情况的研究。
