Costa Larissa Cristina Prado das Neves, Siqueira Jones Anderson Monteiro, Portal Thayara Morais, Sousa Edivaldo Costa, Linhares Alexandre da Costa, Gabbay Yvone Benchimol, Resque Hugo Reis
Programa de Pós-Graduação em Biologia Parasitária na Amazônia, Universidade do Estado do Pará, Belém, PA, Brasil.
Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, PA, Brasil.
Rev Soc Bras Med Trop. 2017 Sep-Oct;50(5):621-628. doi: 10.1590/0037-8682-0198-2017.
Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992.
HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing.
Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected.
The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.
急性胃肠炎（AGE）是发病和死亡的最常见原因之一，在发展中国家的儿童中尤为如此。人腺病毒（HAdV）和札幌病毒（SaV）是导致AGE的病原体。本研究旨在检测1990年至1992年期间在帕拉州贝伦市一个低收入社区进行的监测研究中收集的172份急性胃肠炎患儿粪便样本中的HAdV和SaV，并对其进行基因分型。
使用引物Hex1deg/Hex2deg和NeHex3deg/NeHex4deg通过巢式PCR检测HAdV。通过逆转录PCR（RT-PCR）、巢式PCR和定量PCR检测SaV。通过直接循环测序确定核苷酸序列。
总体而言，43%（74/172）的样本HAdV呈阳性，其中70.3%（52/74）进行了测序并归类为属于五个不同的种，主要是A和F种。对于SaV，阳性率为5.2%（9/172），检测到基因型GI.1、GI.7、GII.1和GV.2。
目前的结果强化了进一步开展研究以获取这些病毒在巴西尤其是亚马逊地区传播的流行病学数据的必要性，该地区20世纪90年代初的数据稀缺。此外，该研究首次描述了在巴西检测到SaV基因型GI.7和GV.2，表明这些类型在该地区25年前就已传播。
