Yao Yongliang, Wu Jianhong, Gu Tao, Cheng Yang, Li Guangxin
Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
Department of Pathology, Chongqing Cancer Institute Chongqing 400030, People's Republic of China.
Am J Transl Res. 2016 Oct 15;8(10):4184-4194. eCollection 2016.
may cause chronic gastritis, even gastric cancer, however, antigen-presenting cells (APCs) are most important immune cells involved in the induction and expression of the underlying inflammatory responses to resist . To study the interaction of HSPs in dendritic cells (DCs), macrophages and RGM-1 cells infected with , HSP-27, HSP-60, HSP-70 and HSP-90 proteins were analyzed in the mucosa tissue or serum of gastritis patients caused by , and in cell supernatant of DCs, macrophages, RGM-1 infected by , or in above host cells. We found that HSP-27, HSP-60, HSP-70 and HSP-90 decreased in gastric epithelial cells, but increased significantly in DCs, macrophages. Meanwhile, inflammation associated proteins iNOS-2 and COX-2 were participated in the expression of HSPs in the process of host cells defensing against infection. These findings contribute to understand the functions of HSP-27, HSP-60, HSP-70 and HSP-90 in infection APCs and gastric epithelial cells indicating that HSPs would be diagnostic markers for infection.
可能导致慢性胃炎，甚至胃癌，然而，抗原呈递细胞（APC）是参与诱导和表达潜在炎症反应以抵抗感染的最重要免疫细胞。为了研究热休克蛋白（HSP）在树突状细胞（DC）、巨噬细胞和感染[病原体名称未给出]的RGM - 1细胞中的相互作用，对由[病原体名称未给出]引起的胃炎患者的黏膜组织或血清，以及被[病原体名称未给出]感染的DC、巨噬细胞、RGM - 1的细胞上清液或上述宿主细胞中的HSP - 27、HSP - 60、HSP - 70和HSP - 90蛋白进行了分析。我们发现，HSP - 27、HSP - 60、HSP - 70和HSP - 90在胃上皮细胞中减少，但在DC、巨噬细胞中显著增加。同时，炎症相关蛋白诱导型一氧化氮合酶2（iNOS - 2）和环氧化酶2（COX - 2）在宿主细胞抵御[病原体名称未给出]感染过程中参与了HSP的表达。这些发现有助于理解HSP - 27、HSP - 60、HSP - 70和HSP - 90在[病原体名称未给出]感染的APC和胃上皮细胞中的功能，表明HSP可能是[病原体名称未给出]感染的诊断标志物。
