视网膜静脉阻塞中基质细胞衍生因子1多态性
Stromal Cell-Derived Factor 1 Polymorphism in Retinal Vein Occlusion.
作者信息
Szigeti Andrea, Ecsedy Mónika, Schneider Miklós, Lénárt Lilla, Lesch Balázs, Nagy Zoltán Zsolt, Fekete Andrea, Récsán Zsuzsanna
机构信息
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
MTA-SE Lendület Diabetes Research Group, Research Laboratory for Pediatrics and Nephrology of the Hungarian Academy of Sciences and of the Semmelweis University, Budapest, Hungary.
出版信息
PLoS One. 2016 Nov 10;11(11):e0166544. doi: 10.1371/journal.pone.0166544. eCollection 2016.
BACKGROUND
Stromal cell-derived factor 1 (SDF1) has crucial role in the regulation of angiogenesis and ocular neovascularisation (NV). The purpose of this study was to evaluate the association between SDF1-3'G(801)A polymorphism and NV complications of retinal vein occlusion (RVO).
METHODS
130 patients with RVO (median age: 69.0, range 35-93 years; male/female- 58/72; 55 patients had central RVO, 75 patients had branch RVO) were enrolled in this study. In the RVO group, 40 (30.8%) patients were diagnosed with NV complications of RVO and 90 (69.2%) patients without NVs. The median follow up period was 40.3 months (range: 18-57 months). The SDF1-3'G(801)A polymorphism was detected by PCR-RFLP. Allelic prevalence was related to reference values obtained in the control group consisted of 125 randomly selected, age and gender matched, unrelated volunteers (median age: 68.0, range 36-95 years; male/female- 53/72). Statistical analysis of the allele and genotype differences between groups (RVO patients vs controls; RVO patients with NV vs RVO patients without NV) was determined by chi-squared test. P value of <0.05 was considered statistically significant.
RESULTS
Hardy-Weinberg criteria was fulfilled in all groups. The SDF1-3'G(801)A allele and genotype frequencies of RVO patients were similar to controls (SDF1-3'A allele: 22.3% vs 20.8%; SDF1-3'(801)AA: 5.4% vs 4.8%, SDF1-3'(801)GG: 60.8% vs 63.2%). The frequency of SDF1-3'(801)AA and SDF1-3'(801)GA genotypes, as well as the SDF1-3'(801)A allele frequency were higher in RVO patients with NV versus in patients without NV complication (SDF1-3'(801)AA+AG genotypes: 57.5% vs 31.1%, p = 0.008; SDF1-3'(801)A allele: 35.0% vs 16.7%, p = 0.002) or versus controls (SDF1-3'(801)AA+AG genotypes 57.5% vs 36.8%, p = 0.021; SDF1-3'(801)A allele: 35.0% vs 20.8% p = 0.01). Carrying of SDF1-3'(801)A allele increased the risk of neovascularisation complications of RVO by 2.69 (OR, 95% CI = 1.47-4.93).
CONCLUSION
These findings suggest that carrying SDF1-3'(801)A allele plays a role in the development of neovascular complications in retinal vein occlusion.
背景
基质细胞衍生因子1（SDF1）在血管生成和眼部新生血管形成（NV）的调控中起关键作用。本研究旨在评估SDF1 - 3'G(801)A多态性与视网膜静脉阻塞（RVO）的NV并发症之间的关联。
方法
130例RVO患者（中位年龄：69.0岁，范围35 - 93岁；男/女 = 58/72；55例为中央RVO，75例为分支RVO）纳入本研究。在RVO组中，40例（30.8%）患者被诊断为RVO的NV并发症，90例（69.2%）患者无NV。中位随访期为40.3个月（范围：18 - 57个月）。通过聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）检测SDF1 - 3'G(801)A多态性。等位基因频率与由125名随机选择的、年龄和性别匹配的无关志愿者组成的对照组中的参考值相关（中位年龄：68.0岁，范围36 - 95岁；男/女 = 53/72）。通过卡方检验确定组间（RVO患者与对照组；有NV的RVO患者与无NV的RVO患者）等位基因和基因型差异的统计分析。P值<0.05被认为具有统计学意义。
结果
所有组均符合哈迪 - 温伯格标准。RVO患者的SDF1 - 3'G(801)A等位基因和基因型频率与对照组相似（SDF1 - 3'A等位基因：22.3%对20.8%；SDF1 - 3'(801)AA：5.4%对4.8%，SDF1 - 3'(801)GG：60.8%对63.2%）。与无NV并发症的患者相比，有NV的RVO患者中SDF1 - 3'(801)AA和SDF1 - 3'(801)GA基因型的频率以及SDF1 - 3'(801)A等位基因频率更高（SDF1 - 3'(801)AA + AG基因型：57.5%对31.1%，p = 0.008；SDF1 - 3'(801)A等位基因：35.0%对16.7%，p = 0.002），或与对照组相比（SDF1 - 3'(801)AA + AG基因型57.5%对36.8%，p = 0.021；SDF1 - 3'(801)A等位基因：35.0%对20.8%，p = 0.01）。携带SDF1 - 3'(
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