斑马鱼胚胎转座酶可及染色质高通量测序分析（ATAC-Seq）实验方案

Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos.

作者信息

Doganli Canan, Sandoval Melissa, Thomas Sean, Hart Daniel

机构信息

Cardiovascular Research Institute, University of California, San Francisco, CA, 94158, USA.

Gladstone Institute of Cardiovascular Disease, San Francisco, CA, 94158, USA.

出版信息

Methods Mol Biol. 2017;1507:59-66. doi: 10.1007/978-1-4939-6518-2_5.

DOI:10.1007/978-1-4939-6518-2_5

PMID:27832532

Abstract

Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is a useful method to map genome-wide chromatin accessibility and nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebrate embryos.

摘要

高通量测序转座酶可及染色质分析（ATAC-seq）是一种用于绘制全基因组染色质可及性和核小体定位的有用方法。全基因组测序利用原核转座酶Tn5插入染色质可及区域的衔接子序列来进行。在这里，我们描述了ATAC-seq在斑马鱼胚胎中的应用，以及该方法在整个脊椎动物胚胎中的适用性。