Wu Jie, Quan Jian Ping, Ye Yong, Wu Zhen Fang, Yang Jie, Yang Ming, Zheng En Qin
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Yi Chuan. 2020 Apr 20;42(4):333-346. doi: 10.16288/j.yczz.19-279.
Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) was developed in 2013. It has the advantages of more convenient operation and higher efficiency for DNA recovery than DNase I hypersensitive site sequencing (DNase-seq) and micrococcal nuclease sequencing (MNase-seq). ATAC-seq currently is the most popular technique of genome-wide mapping for chromatin accessibility. It provides information on binding regions of transcription factors and nucleosome localization on the chromatin. Thus, ATAC-seq is of great significance for studying the epigenetics and molecular mechanisms in chromatin structure. In this review, we compare the advantages and disadvantages of multiple techniques for profiling chromatin accessibility, and summarize the principles, main process, development and applications of ATAC-seq. We hope this review will provide a reference for study of genome-wide mapping for chromatin accessibility, identification of cis-regulatory elements, and dissection of the epigenetic and genetic regulatory networks using the ATAC-seq technology in eukaryotes.
用于转座酶可及染色质的高通量测序（ATAC-seq）技术于2013年开发。与DNA酶I超敏位点测序（DNase-seq）和微球菌核酸酶测序（MNase-seq）相比，它具有操作更便捷、DNA回收效率更高的优点。ATAC-seq目前是全基因组染色质可及性图谱绘制中最流行的技术。它提供了转录因子结合区域和染色质上核小体定位的信息。因此，ATAC-seq对于研究染色质结构中的表观遗传学和分子机制具有重要意义。在本综述中，我们比较了多种染色质可及性分析技术的优缺点，并总结了ATAC-seq的原理、主要流程、发展及应用。我们希望本综述能为真核生物中利用ATAC-seq技术进行全基因组染色质可及性图谱绘制、顺式调控元件鉴定以及表观遗传和遗传调控网络解析的研究提供参考。
